dna固定在纳米颗粒表面的方法.pptxVIP

磷酸化的DNA与TiO2π-π堆积，如单链DNA与石墨烯，碳纳米管静电吸附1、Biotin-DNA2、炔基修饰的DNA及其他生物分子3、NH2-DNA氨基 羧基氨基 氨基氨基 羧基氨基 羧基1）EDC和NHSFor best results in two-step reactions, perform the first reaction in MES buffer (or other non-amine, non-carboxylate buffer) at pH 5-6, then raise the pH to 7.2-7.5 with phosphate buffer (or other non-amine buffer) immediately before reaction to the amine-containing moleculeDCC（N,N′-Dicyclohexylcarbodiimide）氨基-氨基NHS esters:pH 7-91）BS(PEG)n PEG Crosslinkers - Amine Reactive Water2）TSAT (Tris-succinimidyl aminotriacetate)Water3）DSS (disuccinimidyl suberate)Water4）BSOCOES (bis[2-(succinimidyloxycarbonyloxy)ethyl]sulfone) Water5）DSG (disuccinimidyl glutarate)DMSO or DMF6）DTSSP (3,3′-Dithiobis[sulfosuccinimidylpropionate]) Water7）DST (disuccinimidyl tartrate)DMSO or DMF8）BS3 (Bis[sulfosuccinimidyl] suberate) Water9）EGS [ethylene glycolbis(succinimidylsuccinate)]DMSO or DMF 10）Sulfo-EGS [ethylene glycolbis(sulfosuccinimidylsuccinate)]Water4、巯基化的DNA1 Au-S Ag-S2 巯基 氨基3 巯基 羧基4 巯基 羟基5 巯基 巯基巯基-氨基NHS esters:pH 7-9；Maleimides:pH 6.5-7.5；Usually 7.2-7.5。Two steps reaction1）AMAS (N-[α-maleimidoacetoxy] succinimide ester)DMSO or DMF2）BMPS (N-[β-maleimidopropyloxy] succinimide ester)DMSO or DMF3）EMCS (N-[ε-maleimidocaproyloxy]succinimide ester)DMSO or DMF4）Sulfo-EMCS (N-[ε-maleimidocaproyloxy]sulfosuccinimide ester)Water5）GMBS (N-[γ-maleimidobutyryloxy]succinimide ester)DMSO or DMF6）Sulfo-GMBS (N-[γ-maleimidobutyryloxy]sulfosuccinimide ester)Water7）BMPA (N-β-maleimidopropionic acid)EDC，0.1mol MES pH4.7 Maleimides react with sulfhydryls (–SH) at pH 6.5-7.58）LC-SMCC (Succinimidyl-4-[N-maleimidomethyl]cyclohexane-1-carboxy-[6-amidocaproate])DMSO or DMF9）SMPB (succinimidyl 4-[p-maleimidophenyl]butyrate)DMSO or DMF10）Sulfo-SMPB (sulfosuccinimidyl 4-[p-maleimidophenyl]butyrate)Water11）Sulfo-LC-SPDP, LC-SPDP, SPDP巯基-羧基巯基-羟基异氰酸脂在水溶液中很容易水解，所以最好用有机溶剂如DMSO、DMF等。如在水相中，则要使用pH8.5的碱性缓冲溶液，避免使用含羟基的缓冲液如tris缓冲液，且要注意含羟基的分子不能有氨基和巯基，因为pH＞7.5顺丁烯二酰亚胺失去对巯基的特异性，会同时与氨基和巯基发生反应。巯基-巯基pH6.5-7.5表征：1、定性（红外表征，如交联