分光光度法测定血清中尿酸含量_冯晓晶.pdfVIP

National Medical Frontiers of China, Jan 2011, Vol.6 No.1 中国医疗前沿 2011年1月 第6卷 第1期 临床诊断 67 分光光度法测定血清中尿酸含量 冯晓晶 张加玲 【摘要】 目的 建立一种简便、快速测定血清中尿酸含量的方法。方法 尿酸将铁（Ⅲ）还原为铁（Ⅱ），其进一步与邻二氮菲生 成橙红色的配合物，在510nm波长处测定其吸光度值，并详细探讨影响该显色反应的各种因素。结果 在选定的最佳实验条件 下，尿酸含量在 0.25 ～ 7.00mg/L 范围内线性良好，相关系数r =0.9997，精密度RSD＜3.70%，加标回收率 84.0% ～ 101.0%。结 论 该方法用于血清中尿酸含量的测定，结果令人满意。 【关键词】 尿酸；分光光度法；血清；嘌呤 doi:10.3969/j.issn.1673-5552.2011.01.0044 【中图分类号】R-331；R446.11 【文献标识码】A 【文章编号】1673-5552(2011)01-0067-02 Determination of Serum Uric Acid by Spectrophotometry FENG Xiao-jing, ZHANG Jia-ling. Department of Hygienic Inspections, School of Public Health, Shanxi Medical University, Taiyuan 030001, China 【Abstract】Objective A simple and rapid method for the determination of uric acid in serum was proposed. Methods The Fe (Ⅲ) was reduced to Fe(Ⅱ) by uric acid, which then formed an orange complex with phenanthroline. The absorbance of the complex was measured at a wavelength of 510nm, and the optimal extraction and reaction conditions were studied. Results Under the optimum conditions, linearity was excellent in the range of 0.25-7.0mg/L, correlation coefficient r=0.9997. The coefficient of variation in this method was less than 3.70%, and the recoveries were in the range of 84.0%-101.0%. Conclusion The proposed method was applied to the determination of uric acid in human serum sample with satisfactory results. 【Keywords】Uric acid; Spectrophotometry; Serum; Purine 随着生活水平的不断提高，人们膳食结构的变化，高嘌呤类 恒温水浴箱（北京医疗设备厂）。 食物如海鲜、肉类等的摄入量明显增加，而长期摄入富含嘌呤类 1.2 主要试剂 的食物再加上一些诱导因素可引起嘌呤代谢异常，从而导致嘌 1.2.1 尿酸标准储备液(1.0000g/L)：准确称取0.1000g尿酸， 呤代谢的终产物尿酸(2,4,6-三羟基嘌呤,简称UA)在体内沉 溶于6ml 0.1mol/L氢氧化钠溶液中，用蒸馏水定容至100ml。 积。正常人血浆中尿酸含量约为2.0 ～ 6.0mg/dL，男性平均为 1.2.2 尿酸标准应用液（0.05g/L）：由尿酸标准储备液稀释得到。 4.5mg/dL，女性平均为3.5mg/dL。正常含量的尿酸对机体是有 1.2.3 铁（Ⅲ）储备液(0.30g/L)：准确称取0.2590g硫酸铁