e8微生物学南京师范大学英文版.pptxVIP

MICROBIOLOGY Chapter 8. Microbial Genetics by Weilan Shao; Definition review Genetics is the science of heredity; it is concerned with the physical and chemical properties of the hereditary material, how this material is transmitted from one generation to the next, and how the information it contains is expressed during the development of an individual. ---- Stanley R Maloy et al., in MICROBIAL GENETICS, 1994 ;MICROBIOLOGY Chapter 8. Microbial Genetics IV. Applications of Microbial Genetics;Main topics: BREEDING BY INDUCED MUTATION BREAKTHROUGHS LEADING TO RECOMBINANT DNA TECHNOLOGY STRATEGIES OF USING RECOMBINANT DNA TECHNIQUES PRODUCTIONS OF RECOMBINANT PROTEINS GENETIC MODIFICATION OF ORGANISMS;Main topics: BREEDING BY INDUCED MUTATION BREAKTHROUGHS LEADING TO RECOMBINANT DNA TECHNOLOGY STRATEGIES OF USING RECOMBINANT DNA TECHNIQUES PRODUCTIONS OF RECOMBINANT PROTEINS GENETIC MODIFICATION OF ORGANISMS; A “classical” genetic technique for strain breeding Physical agents Mutagenic agents e.g. X-rays, g-rays, UV Chemical mutagens e.g. base analogs, nitrous acid, alkylating, arylating agents;;;;Advantages Industrial strain can be improved to a good level of production before related genetic information is obtained. This is very important for fermentation of metabolites, especially for 2nd metabolites.;Disadvantages Relatively minor modifications can be obtained. The nature of mutations is not known.;Main topics: BREEDING BY INDUCED MUTATION BREAKTHROUGHS LEADING TO RECOMBINANT DNA TECHNOLOGY STRATEGIES OF USING RECOMBINANT DNA TECHNIQUES PRODUCTIONS OF RECOMBINANT PROTEINS GENETIC MODIFICATION OF ORGANISMS;An overview Biotechnology has been greatly advanced by the introduction of recombinant DNA technologies because organisms can now be greatly modified to accomplish goals that were previously impossible. ---- Kathleen P Talaro, in FOUNDATIONS IN MICROBIOLOGY,