蛋白组学在肾脏病研究中应用.ppt

蛋白组学在肾脏病研究中的应用;前言;蛋白组学与肾脏;内 容;蛋白质组和蛋白质组学;Proteomics includes not only the identification and quantification of proteins, but also the determination of their localization, modifications, interactions, activities, and, ultimately, their function.;Nephrol Dial Transplant (2010) 25: 11–16;研究过程：蛋白质分离、鉴定以及信息资料分析处理三部分;蛋白质组学重要工具;Two-dimensional Gel Electrophoresis;;质谱技术;表面增强激光解吸电离飞行时间质谱（surface enhanced laser desorption ionization time-of flight mass spectrometry， SELDI-TOF-MS） 通过???量肽段离子相关参数,如飞行时间(TOF),即可计算得到准确的肽段质量 ;Four different types of MS-based proteomic technologies (a) 2DE-MS; (b) SELDI-MS;