动力学亲和力分析.pptVIP

* * This slide shows how the terms in the net rate expression relates to the sensorgram. Rmax is the maximum level that analyte binding can generate. All target/ligand sites are occupied. This is indicated in the sensorgram generated by the injection of a higher concentration of analyte. At each time point, the concentration of free/unoccupied ligand can be expressed as Rmax-R dR/dt or the change in response over time is given by the slope of the curve. * * The sensorgram contains information about association and dissociation rates, and also equilibrium levels (strictly steady state) if a pla