第七章抗原抗体反应与应用.pptVIP

第七章 抗原抗体反应及应用;第一节 抗体的制备;一、抗血清的制备;二、抗体的特性;抗原抗体作用力;2 抗体的特性测定; 亲和力是单个抗原表位与单个抗体结合位点之间的作用力，反映抗体与抗原表位特异性结合强弱的量化指标。;;亲合力（Avidity）p156;3 影响抗原-抗体反应的因素;Polyclonal antibodies can form lattices, or large aggregates, that precipitate out of solution. If each antigen molecule contains only a single epitope recognized by a given monoclonal antibody, the antibody can link only two molecules of antigen and no precipitate is formed.;第二节 抗原抗体反应原理;;抗体结合抗原的特异性;;;;交叉反应;第三节 常见免疫分析方法;A precipitation curve for a system of one antigen and its antibodies;;1 溶液中的沉淀反应 环状沉淀试验;在凝胶或琼脂中形成可见的沉淀线;单向扩散 (radi