BCA法测蛋白含量.docVIP

The Colorimetric Detection and Quantitation of Total Protein Randall I. Krohn Pierce Biotechnology Inc., Rockford, Illinois Current Protocols in Cell Biology Published online September 2011 in Wiley Online Library THE BICINCHONINIC ACID (BCA) ASSAY FOR DETERMINATION OF TOTAL PROTEIN Principle原理 Smith et al. (1985) introduced the bicinchoninic acid (BCA) protein assay reagent. In one sense, it is a modification of the Lowry protein assay reagent. The mechanism of color formation with protein for the BCA protein assay reagent is similar to that of the Lowry reagent, but there ar