深度解密lncRNA研究策略及注意事项.pdfVIP

深度解密lncRNA研究策略及注意事项 张晓娜 上海伯豪生物技术有限公司 共表达网络构建 高通量转录组检测对象 RNA transcripts Protein-coding RNA(mRNA) Non-coding RNA(非编码RNA) Regulatory RNAs Housekeeping RNAs microRNA lncRNA circRNA 2 LncRNA 概述 ——lncRNA 的发现 1980s-1990s: Individual lncRNAs are discovered through traditional gene mapping approaches, Xist and H19. Early 2000s: Development of large scale cDNA sequencing leads to the discovery of a surprising number of lncRNA transcripts. Mid 2000s: The number of predicted genes in the mammalian genome goes down and the number of detected lncRNA transcripts increases exponentially. 国自然资助lncRNA项目数量逐年上升 LncRNA基本特点 • 200nt • have little or no open reading frame (ORF) • Polyadenylated are not necessary (more than 40% of transcripts are non-polyadenylated) • Generally lower expressed than protein-coding genes • more tissue-specific expression patterns, with a large fraction of tissue-specific lncRNAs expressed lncRNA同时在细胞核和细胞质内发挥功能 in the brain. http://www.rikenresearch.riken.jp/eng/frontline/5920 • general long ncRNAs lack strong conservation lncRNA 的功能 Magdalena Losko, Jerzy Kotlinowski, and Jolanta Jura, 2016, Mediators of Inflammation lncRNA 的类别 Knauss and Sun, Neuroscience 2013 lncRNA研究策略 组织，细胞，外周血 lncRNA芯片 RNA-seq 难点  差异倍数大的  表达量高的