Nisepharose6FastFlow英文使用说明书.pdfVIP

GE Healthcare Instructions 11-0008-87 AE Affinity media Caution! Contains nickel. Please read these instructions carefully before use. May produce an Pay extra attention to the chapters “General considerations ” and “Preparation before purification allergic reaction. Ni Sepharose? 6 Fast Flow Immobilized metal ion affinity chromatography (IMAC) exploits the interaction between chelated transition metal ions and side-chains of certain amino acids (mainly histidine) on proteins. In general, Ni 2+ is the preferred metal ion for purification of histidine-tagged proteins. The IMAC medium Ni Sepharose 6 Fast Flow consists of 90 μm beads of highly cross-linked agarose, to which a chelating group has been coupled. This chelating group has then been charged with nickel (Ni 2+ ) ions. Ni Sepharose 6 Fast Flow has low Ni 2+ leakage, high protein-binding capacity, and is compatible with a wide range of additives used in protein purification. Its high flow properties make it excellent for scale-up. The medium is also suitable for gravity flow column purification and multiwell plate screening. Ni Sepharose 6 Fast Flow is available in 5, 25, 100 and 500 ml lab packs, prepacked in 1-ml His GraviTrap? gravity-flow columns and HisTrap? FF 1 and 5-ml, HisTrap FF crude 1 and 5-ml and 20-ml HisPrep? FF 16/10 columns for use in chromatography system such as ?KTA? design or FPLC? System. Table of contents Product description 2 General considerations 5 Column packing 6 Preparation before purification 8 Purification procedure for a packed column 11 Batch/gravity-flow purific