MdMYB 111和MdWRKY40参与红肉苹果花青苷生物合成的分子机制.pdfVIP

符号中英文缩略说明 Amp: Ampicillin, 氨苄青霉素 ACE: Acetosyringone, 乙酰丁香酮 bp: Base pair, 碱基对 Cb: Carbenicillin, 羧苄青霉素 cDNA: Complementary DNA, 互补DNA CDS: Coding domain sequence, 基因编码序列 ddH O: Distilled and deionized water, 重蒸水 2 DMSO: Dimethyl sulphoxide, 二甲基亚砜 DNA: Deoxyribonucleic acid, 脱氧核糖核酸 dNTP: Deoxy ribonucleoside triphosphate, 脱氧核糖核苷三磷酸 IPTG: Isopropylthio-β-D-galactoside, 异丙基-β-D-硫代半乳糖苷 IAA: Indole-3-acetic acid, 吲哚乙酸 Kan: Kanamycin, 卡那霉素 LB: luria-bertani medium LB, 培养基 MS: Murashige and Skoog media MS, 培养基 OD: Optical density, 光密度 PBS: Phosphate buffer solution, 磷酸缓冲液 PMSF: Phenylmethanesulfonyl fluoride, 苯甲基磺酰氟 PCR: Polymerase chain reaction, 聚合酶链式反应 rpm: Revolutions per minute, 转/分钟 SDS: Sodium dodecyl sulfate, 十二烷基硫酸钠 TE: Tris·Cl, EDTA buffer, Tris·Cl、EDTA 缓冲液 TEMED: N,N,N,N-Tetramethylethylenediamine, 四甲基乙二胺 TDZ: Thidiazuron, 噻苯隆 Tris: Tris (hydryxymethy) laminomethane, 三羟甲基氨基甲烷 X-gal: 5-Bromo-4-chloro-3-indolyl-β-Dgalactoside, 5-溴-4-氯-3-吲哚-β-D-半乳糖苷 YEP: Yeast extract and peptone medium, YEP 培养基 2,4-D: 2, 4-Dichlorophenoxyacetic acid, 2,4-二氯苯氧乙酸 6-BA: N-(Phenylmethyl)-9H-purin-6-amine, 6-苄氨基嘌呤 目录 中 文 摘 要 I Abstract III 1 引 言 1 1.1 植物花青苷研究进展 ················································································ 1 1.1.1 花青苷的结构和种类 ············································································· 1 1.1.2 花青苷的功能 2 1.2 花青苷的生物合成 ·················································································· 3 1.2.1 花青苷的合成途