抗体及其基因b细胞发育englishversion图文.pptxVIP

Antibody Molecules,Their Genes and B Cell Development ;ANTIBODY 抗体;NOBEL PRIZES FOR ANTIBODY RESEARCH;7-1;DEFINITION?: Antibody (Ab) A globulin which is produced by plasma cell as a result of the introduction of an antigen and which has the ability to combine with the antigen that stimulated its production.;DISTRIBUTION OF ANTIBODY: Antibody molecules are found in serum, (account for approxinmately 20% of the total plasma pritein ),in extravascular Fluids（血管外液体）, in exocrine secretions(外分泌液）, and on the surface of some lymphocytes.;;Figure 1 Electrophoretic separation of serum proteins;7-2 抗体分子的基本结构 Basic Structure of Ab Molecules ;Rodney Porter 1917-1985 Nobel Prize in 1972;;;;Figure 2 The basic structure of immunoglobulins;1.Heavy light chains 1)Heavy chain: ① composed of about 500 a.a, oligosaccharide(+) ② Class : heavy chain ?, ? , ? , ?, ?. immunoglobulin(Ig) IgA,IgG, IgM IgD, IgE 2) light chain: ① composed of about 214 a.a, oligosaccharide(-) ② type: ? ,? ;;;;6.joining chain secretory piece;;;7-4 Properties Biological Activities of Five Classes of Immunoglobulins;;;2. Properties IgG is the major Ig in serum - 75% of serum Ig is IgG The longest half life(t ? =23days) IgG is the major Ig in extravascular spaces d) Placental transfer e) Fixation complement - Not all subclasses fix equally well; IgG4 does not fix complement f) Binding to cells – Macrophages, monocytes,.- Opsonization. NK cell, Macrophages---mediating ADCC SPA—Coagglutination Test;;IgG molecule;1.structure presence ;2. Properties the 3rd highest serum Ig the first Ig made by fetus and B cells intravascular Ig d) Fixation complement –classical pathway e)the largest size of molecule f)agglutinating microbes e) Binding to cells – Macrophages, monocytes,.- Opsonization. NK cell, Macrophages---mediating ADCC ;;;;;;;;;表; a u