不同株型玉米籽粒胚乳细胞与淀粉.docxVIP

不同株型玉米籽粒胚乳细胞与淀粉合成关键酶活性分析 ［摘 要］【目的】 研究不同类型玉米品种籽粒胚乳细胞增殖与籽粒充实期淀粉合成关键酶的关系。 【方 法】以紧凑型玉米品种（先玉335和郑单958）和平展型玉米品种（长城799和农大364）为试验材料，于授粉 后3，5，7，10，15 d等取果穗中部籽粒，测定胚乳细胞数、单粒质量、淀粉含量、籽粒 ADP审UDPG焦 磷酸化酶活性、可溶性淀粉合成酶（SSS）和束缚态淀粉合成酶（GBSS）活性，研究不同玉米品种籽粒胚乳细胞 增殖动态和籽粒相关酶活性的关系。 【结果】在灌浆中后期，先玉335籽粒淀粉合成关键酶活性均高于其他 3个品种；先玉335和郑单958籽粒胚乳细胞数、淀粉含量、单粒质量显著高于长城 799和农大364。玉米 籽粒胚乳细胞数与粒重、淀粉含量和四种淀粉合成关键酶均呈正相关。 【结论】库容量和库活性是制约玉米 籽粒淀粉积累的主要因素。保证籽粒充实期间较高的胚乳细胞增殖速率及淀粉合成关键酶活性是玉米实现 高产的关键。 ［关键词］玉米；胚乳细胞；焦磷酸化酶；淀粉合成酶 ［中图分类号］［文献标识码］［文章编号］ Relationship between endosperm cell and activities of key enzymes for starch in maize with different plant type 1a 1b 1b 2 1a 1b GUYan，HU Wen-he， WAN（Si-yuan ，LIANG Xuan-he， HEWen-an ， Sun Ming-chun , WU Chun-she ng1a （1.a The Research Center of Crop, b. College of Agronomy ，Jilin Agricultural University ， Changchun Jilin 130118 ； 2. Academy of Agricultural Sciences of Jilin Provinee, Changchun ，Jilin130124,China） Abstract :【Objective 】The proliferation process of endosperm cell and its relationship with activities of key enzymes for starch were studied. 【Method】The upright maize varieties Xianyu 335 and Zhengdan 958 and the common maize varieties Changcheng 799 and Nongda 364 were chosen as the expeiriment material. Central kernels were collected after the pollination stage, endosperm cell number, kernel weight, starch content, activities of key enzymes for starch were determined, and the relationship was analyzed. 【Result 】 During the later grain-filling period, key en zymes for starch of Xia nyu 335 were higher tha n other three varieties. And the en dosperm cell nu mber, starch content, gra in weight of iany u335 and Zhen gda n 958 were higher tha n Chan gche ng 799 and Non gda 364. The en dosperm cell nu mber had significantly positive correlation with grain weight, starch content and the enzyme activities for starch. 【Conclusion 】 The results here suggested that grain sink strength and activities are the main factor responsible for starch accumulation in maize. The higher en dosper