层析介质比较.docxVIP

疏水层析介质特性比较 Table L Media characteristics Degree of substitution Ligond density per ml medium Pheny l Sepharose 6 Fast Flow low sub 25 mol Phenyl Sepharose 6 Fast Flow high sub 40 pmol Butyl-S Sepharose 6 Fast Flow 10 pmol Bjtyl Sepharose 4 Fast Flow 40 pmol Octyl Sepharose 4 Fast Flow 5 pmol Mean particle size 90 pm Bead size range 45-165 pm Bead structure Spherical highly cross-linked agarose, 6 or 4% Max volumetric flow rate (XK16/201 Sepharose 6 Fast Flow 15 ml/min Sepharose 4 Fast Fllow 8 ml/min Max linear flovu rate Sepharose 6 Fast Flow 450 cm/hour Sepharose 4 Fast Flow 240 cm/hour 分子筛介质： 4 Fa^t Fkkw Sepharoe 6 Fast ftow ￡kcIj^ on liml for^labulo^proteins = 3 n 10* IO fof dextrans ? IO ~ ?k 1(X Average particle see 90 90 pm Parlcole size rarige 4S la 165 pm to 165 pm Bead structu re highly cross- h nked aga rose, 4% high g CToss-fcn ked ogarose. 6% Flew welQcjt^cit JOO kPa, ^S*C s 25flcrrVh s ZdOem/h (XK 50/3D cokimn, 15 cm bed height) pH wtgiliTy working rm昨 ?to 32 ? to 12 deaniKig- ki-pksc# ￡ to M 7 to 14 Chemict]l for 7 d| ? M Na OH, 7M4 fttianal 30% isogwopanol 30% aceto nilrie. 1%SD5.2%dupto. 6 F guanidki^hy^rochktride. 8 M AuToEhuabte 121*C ftr 20 mn in H?O 121*C for 20 mln in H^O Storage 20% ethcrwl at 4 to 30*C ?0% ethenol at 4 to 3O*C 产品 IE?檄置口〕 WiJi 持ft J匝用 pH程建性 耐压 酩兵派席 工作沽浏 Ef倾 (cnVhl 四]Spphornse Flow ?有寺怪明(科映琼脂＜tn■质，大犬jh迷了利搪怯散 读恃快.适合工叩现愫牛产. ?冬去电荷处氏.等特异性嘴咐峻低.提肉回氏率一 ?相身迥化学稳定件＜ 可用裂初.有机寿郝工作瓦卜出NQH在位就流， 17-DL59-G1 itoYtitWitifr IL 10,004 ir-DlSKfi IQL as ifi? 巨云竹耳如口心助驻 17-01^9-01 Se^hcnreeiff IL ￡0,000- “T5 巨大分于虬重军乙空蜀孟 1.T-DL49-? 1OL 如“ JH日挤廉.璃毒 2-15 OU 250 离子交换： Table 1, HiTrap SP HP and HFTrap Q HP columns characteristics Column volumes Column dimen^iors Total ionit capacity Dynamic binding capacity Mean particle size Betid structure Maxinnim flow rotes Recommended flow rates Maximijm Chemical stability Charged group 1 ml or 5 ml 0.7 x 2 5 cm (1 ml I and 1.6 x 2.5 cm 15 ml! 0.1^-020 mnnol ICWml medium IQ) 015-Ct20mmol [H J/ml medium(州 SP: approx, 55