16. 叶原基离体培养和活体成像.docxVIP

04/01/2017 Bihai Shi Page 3 of 3 Live imaging of axillary meristem This protocol can be used for in vitro culture and live imaging of leaf promordia of Arabidopsis thaliana. Reference for the protocol: Shi B, Zhang C, Tian C, et al. (2016) Two-Step Regulation of a Meristematic Cell Population Acting in Shoot Branching in Arabidopsis. PLoS Genet. 12(7):e1006168. I. Materials FM4-64 (Life technologies, Cat Not T3166/T13320, work conc.: 50 μg/ml) Autoclaved ddH2O Fine forceps Hypodermic sterile insulin syringes Dissecting micro