重金属胁迫下铜绿微囊藻的变化特点分析-植物学论文-生物学论文.docxVIP

重金属胁迫下铜绿微囊藻的变化特点分析-植物学论文-生物学论文 ——文章均为WORD文档，下载后可直接编辑使用亦可打印—— 　　摘 要：　为了明确藻类对重金属的耐受性和富集特征, 采用室内培养法, 研究铜绿微囊藻 (Microcystis aerugino-sa) 对不同质量浓度Zn2+和Cd2+的富集能力以及对其胁迫的生理响应。结果表明, 铜绿微囊藻对Zn2+、Cd2+均有一定的富集作用, 而且Zn2+的富集量明显高于Cd2+。当Zn2+质量浓度为0. 05 mg/L时, 铜绿微囊藻的比生长速率最快, 当Zn2+质量浓度超过0. 05 mg/L时, 铜绿微囊藻的生长受到抑制, 即Zn2+对铜绿微囊藻的生长具有低质量浓度促进, 高质量浓度抑制的作用。Cd2+对铜绿微囊藻的生长不具有低质量浓度促进作用, 质量浓度为0. 05 mg/L的Cd2+作用96 h后, 铜绿微囊藻的生长受到明显抑制, Cd2+质量浓度越高, 其对铜绿微囊藻生长的抑制作用越明显。Zn2+、Cd2+质量浓度分别为0. 20 mg/L和0. 15 mg/L时, 连续培养24 h后, 铜绿微囊藻的酯酶活性均显着升高, 当Zn2+、Cd2+质量浓度分别达到0. 20 mg/L和0. 25 mg/L时, 与对照相比, 藻细胞光系统II (PSII) 最大光能转化效率 (Fv/Fm) 显着降低。在Zn2+、Cd2+胁迫下, 铜绿微囊藻的超氧化物歧化酶 (SOD) 活性、过氧化氢酶 (CAT) 活性以及丙二醛 (MDA) 含量均发生变化。 　　关键词：　铜绿微囊藻; 锌; 镉; 生理响应; 富集能力; 　　Abstract：　In order to study the tolerance and enrichment characteristics of algae to heavy metals, the enrichment ability and physiological response of Microcystis aeruginosa to different concentrations of Zn2+ and Cd2+ were investigated by using indoor culture methods. The results showed that Microcystis aeruginosa had a certain enrichment effect on Zn2+ and Cd2+ , and the enrichment of Zn2+ in Microcystis aeruginosa was obviously higher than that of Cd2+ . Microcystis aeruginosa grew fastest when the concentration of Zn2+ was 0. 05 mg/L, while the growth was inhibited when the concentration of Zn2+ exceeded 0. 05 mg/L. The low concentrations of Zn2+ could promote the growth of Microcystis aeruginosa, while high concentrations could inhibit its growth, which was called the hormesis effect. However, the growth of Microcystis aeruginosa was not promoted by low concentration of Cd2+ and was obviously inhibited after being treated with 0. 05 mg/L Cd2+ for 96 h. With the increase of Cd2+ concentration, the inhibitory effect of Cd2+ on the growth of Microcystis aeruginosa became more and more obvious. When the concentration of Zn2+ and Cd2+ reached 0. 20 mg/L and 0. 15 mg/L, the esterase activity of Microcystis aeruginosa increased significantly after continuous culture for 24 hours.