系列烷基甲基萘磺酸盐的高效液相色谱和电喷雾离子化质谱分析.docxVIP

Vol. 24 No. 3 10~ 13 系列烷基甲基萘磺酸盐的高效液相色谱和电喷雾离子化质谱分析赵忠奎，乔卫红，付新梅，李宗石，程侣柏 (大连理工大学精细化工国家重点实验室，辽宁大连116012) 摘 要：采用反相离子对高效液相色谱 (HPLC)和电喷雾离子化质谱 (ESI - MS)对系列长链烷基甲基萘磺酸盐 (AMNS),包括己基甲基萘磺酸盐 、辛基甲基萘磺酸盐、癸基甲基萘磺酸盐、十二烷基甲基萘磺酸盐 、十四烷基甲 基萘磺酸盐进行分析，通过液相色谱分离条件的优化 ，找到了最佳分析条件。紫外检测波长230 nm ,流动相为 甲醇和水混合物，流速为0. 8 mL/ mi n ,采用梯度洗脱方式进行分析 。线性梯度：0~40 m n时甲醇-水体积为 60 ：40 ,在40~ 70 mi n时，甲醇-水体积比为100 ：0。样品分离度高，分离效果较好。用面积归一化法得到了 样品各组分的相对百分含量 。HPLC和ESI - MS是快捷、有效和可靠的分析和表征 AMNS的方法。 关键词：高效液相色谱；电喷雾离子化质谱；烷基甲基萘磺酸盐；表征 中图分类号：0657. 72 ; 0657.63 文献标识码：A 文章编号：1004 - 4957 (2005) 03 - 0010 - 04 HPLC and ESI - MS Characterization of Al kyl methy nap hthale ne Sulfo nates ZHAO Zhong- kui , QIAO Wei- hong , FU Xin- mei , LI Zong- shi , CHENG L-ubai(State ley Laboratory of F ne Chemical , Dalian University of Technology , Dalian 116012 , China) Ab stract : A series of long chain alkyl methyl naphthalene sulfonates(AMNS , includi ng hexyl methyl naphtha2 lene sulfonate, octyl methyl naphthalene sulfonate , decyl methyl n印hthalene sulfonate , dodecyl methyl naphtha2 lene sulfonate , and tetradecyl methylnaphthalene sulfonate , was characterized by high performanee li quid chro2 matograp hy HPLC) and electrospray ionization mass spectronetry ( ESI - MS) . Through a great deal of test2 I1 i ng , the analytical conditions were opti mized. Detection was carried out at the w avelength of 230 nm. Differ2 ent composition mixtures of methanol and water were used as eluents with a mobile phase flow rate of 0. 8 mL/ mi n. The li near gradient elution was as follows : the volume ratio of methanol to water was 60 : 40 from 0 to 40 mi n and 100 : 0 from 40 to 70 mi n. By using the opti mal analytical conditions , the target composi2 tions were sqoarated satisactoril y. The relative amounts of the constituents were determ ned by an area normal2 ization method. HPLC and ESI - MS are rapid , effective and reliable for the analysis of AM NS. Key wo rds : HFLC ; ESI - MS ; Al kyl methyl n aphthale ne sulfo nates ;Characterization 烷基甲基萘磺酸盐是一