柑橘液胞型焦磷酸质子泵基因鉴定及其在蔗糖和柠檬酸积累调控中的功能验证.pdfVIP

+ Identification ofCitrusVacuolarH -Pyrophosphatase GenesandTheir Function in theRegulation of Sucrose and CitrateAccumulation TableofContents 摘要I ABSTRACTIII CHAPTER 1:INTRODUCTION1 1.1 RESEARCH RATIONALE1 1.2 RESEARCH ADVANCES IN SUGAR AND CITRIC ACID ACCUMULATION IN THE CITRUS FRUIT2 1.2.1 SugarAccumulationin the CitrusFruit2 1.2.2 CitricAcidAccumulation in CitrusFruit7 1.2.3 The ConnectionbetweenSugarand OrganicAcidMetabolism14 1.3 RESEARCH ADVANCES IN VACUOLAR PROTON PUMPS15 1.3.1 The V-ATPase17 + 1.3.2 TheP-typeH -ATPase19 1.3.3 The V-PPase21 1.4 RESEARCH OBJECTIVES,CONTENT,AND SIGNIFICANCE25 CHAPTER2:IDENTIFICATION,CLONINGAND MOLECULAR + CHARACTERIZATION OFCITRUSVACUOLAR H -PYROPHOSPHATASE GENES26 2.1 INTRODUCTION26 2.2 MATERIALS AND METHODS27 2.2.1 PlantMaterial27 2.2.2 RNA ExtractionandcDNA Synthesis27 2.2.3 GeneIsolationandSequencing28 2.2.4 BioinformaticsAnalysis29 2.2.5 Full-lengthSequence Cloning of Putative Genes29 + 2.2.6 SubcellularLocalizationof Citrus VacuolarH -Pyrophosphatase Genes..30 2.2.7 GeneExpressionAnalysis30 2.2.8 StatisticalAnalysis31 2.3 RESULTS31 + 2.3.1 Identificationof Citrus VacuolarH -Pyrophosphatase Genes31 + 2.3.2 SubcellularLocalizationof Citrus VacuolarH -Pyrophosphatase Genes..34 + 2.3.3 SpatialExpressionAnalysis of Citrus TypeI