食品分析(英文课件)：Molecular Fluorescence Spectroscopy.pptVIP

Molecular Fluorescence Spectroscopy Key points： Principle of Fluorescence spectroscopy Qualitative and quantitative methods The composition of the fluorescence spectrophotometer The difference between Fluorescence spectrophotometry and UV spectrophotometry UV I0 I A∝C F (S=10-9~10-12g/ml) Sample UV-vis (LOD=10-6~10-7g/ml) F∝C Fluorescence Parallel to the incident light perpendicular to the incident light （Absorption） （Emission） Brief introduction about fluoremetry Photoluminescence Categories Analysis object Exciting light wave length scope atomic fluorescence analysis Molecular fluorescence analysis UV-Vis fluoremetry Infrared fluoremetry X ray fluoremetry Pathway of Excited molecules return to ground state vibrational relexation internal conversion fluorescence external conversion intersystem crossing Phosphorescence Jablonski diagram Principle of Molecular fluorescence analysis Fluorescence is a photoluminescence process in which molecules are excited by absorption of electromagnetic radiation(excitation light). Collisions with other molecules cause the excited molecule to lose vibrational energy until it reaches the lowest vibrational state of the excited electronic state. The excited species, then relax to the ground state, giving up their excess energy as photons(emitted light). Atomic Fluorescence Spectroscopy (AFS) For atomic species, the process is similar, however since atomic species do not have vibrational energy levels, the emitted photons are often at the same wavelength as the incident radiation. This process of re-emitting the absorbed photon is resonance fluorescence and while it is characteristic of atomic fluorescence Techniques are useful in other kinds of analysis/measurement of a compound present in air or water, or other media, such as CVAFS which is used for heavy metals detection, such as mercury. Relation between fluorescence and molecular structure Rhodamines Strong absorption to UV Most ?uorescent compounds are aromatic. ar