食品分析(英文课件)：GAS CHROMATOGRAPHY.pptVIP

Methods Conditions which can be varied to accommodate a required analysis include: the columns stationary phase, diameter and length inlet temperature detector temperature column temperature and temperature program carrier gas and carrier gas flow rates inlet type and flow rates sample size injection technique chromatographic condition operating conditions separation condition: column Flow rate of carrier gas Column temperature Condition of sample injection Detector The selection of chromatographic column The main chemical attribute regarded when choosing a column is the polarity of the mixture. The polarity of the sample must closely match the polarity of the column stationary phase to increase resolution and separation while reducing run time. The separation and run time also depends on the film thickness (of the stationary phase), the column diameter and the column length. Tavella et al detection of TFA in 46 popular Argentina foods column： CP Sil 88 (50m×0.25mm) Supelco Omegawax 250（30m ×0.25 mm，0.1μm） chromatographic condition： inlet temperature: 250℃, FID: 250℃， column temperature: 185℃（3min）－3℃/min－230℃，M.P. He，column pressure: 19 psi， split ratio: 1：70 Results film thickness (of the stationary phase), the column diameter and the column length e.g.60m × 0.25 mm, 0.2μm length： Increase the column length is good for separation of mixture, but the retention time increased accordingly. 15m：rapid detection simple analyte 25~30m：most commonly used overlength（50、60、100m）：complex compound The selection of chromatographic column Column diameter small diameter: analysis speed Resolution , but column capacity easier to be overload Increase diameter: column efficiency 0.15mm/0.22mm/0.25mm -split sample injection 0.32mm/0.53mm Thickness 0.25~0.5μm， Most widely used Thick film：Retention time Thin film： Temperature Retention time , suitable for high boiling point, thermal-sensitive compounds Column Slection Pretreatment of new column o