酶法测定血清甘油三酯能否消除游离甘油的影响..docxVIP

酶法测定血清甘油三酯能否消除游离甘 油的影响 冯仁丰 黄晓岚 摘要 现有酶法测定试剂不能去除血清中的游离甘油，使甘油三酯值偏 高。尽管反应系统中不存在脂蛋白脂酶，但随着样品和反应系统预孵育时间的 延长，再加入脂蛋白脂酶后，甘油三酯的测定值逐渐下降。说明现有酶法测定 系统不能扣除血清中游离甘油对甘油三酯测定的影响。 关键词 甘油三酯 游离甘油 酶法 Can the free glycerol interference be eliminated in engymatic determination of serum triglycerides Feng Renfeng,Huang Xiaolan ( Shanghai Centre for Clinical Laboratory,Shanghai.200052. ) It is demonstrated that the free glycerol interference cannot be eliminated accurately in current enzymatic analysis of serum triglycerides. In our experiments, the time for incubating serum and the first reagent which was short of lipopr