印度昌迪加尔市微生物技术研究所生物信息学课件fun_prot.pptVIP

Protein Functional Annotation Dr G.P.S. Raghava Annotation Methods Annotation by homology (BLAST) requires a large, well annotated database of protein sequences Annotation by sequence composition simple statistical/mathematical methods Annotation by sequence features, profiles or motifs requires sophisticated sequence analysis tools Annotation by Subcellular localization requires computational tools for better subcellular localization prediction. Annotation by Homology Statistically significant sequence matches identified by BLAST searches against GenBank (nr), SWISS-PROT, PIR, ProDom, BLOCKS, KEGG, WIT, Brenda, BIND Properties or annotation inferred by name, keywords, features, comments Annotation Methods Annotation by homology (BLAST) requires a large, well annotated database of protein sequences Annotation by sequence composition simple statistical/mathematical methods Annotation by sequence features, profiles or motifs requires sophisticated sequence analysis tools Annotation by Subcellular localization requires computational tools for better subcellular localization prediction. Annotation by Composition Molecular Weight Isoelectric Point UV Absorptivity Hydrophobicity Annotation Methods Annotation by homology (BLAST) requires a large, well annotated database of protein sequences Annotation by sequence composition simple statistical/mathematical methods Annotation by sequence features, profiles or motifs requires sophisticated sequence analysis tools Annotation by Subcellular localization requires computational tools for better subcellular localization prediction. Feature based annotation PROSITE - http://www.expasy.ch/ BLOCKS - / DOMO - biogen.fr/services/domo/ PFAM - PRINTS - http://www.biochem.ucl.ac.uk/bsm/dbrowser/PRINTS SEQSITE - PepTool Annotation Methods Annotation by homology (BLAST) requires a large, well annotated database of protein sequences Annotation by sequence composition simple statistical/mathematical methods Annotation by s