黄花蒿挥发油对赤拟谷盗成虫和幼虫的生物活性研究.docxVIP

PAGE 1 黄花蒿挥发油对赤拟谷盗成虫和幼虫的生物活性研究 程 昉 邵亚洲 杨盈盈 刘芯莹 王婧婧 贾凌云 梁俊玉*基金项目：甘肃省自然科学基金（ 基金项目：甘肃省自然科学基金（18JR3RA092） 收稿日期：2020-02-06 作者简介：程昉，男，1966年出生，实验师，天然产物研究 通信作者：梁俊玉，男，1978年出生，副教授，天然产物研究 （ 西北师范大学生命科学学院，兰州 730070） 摘 要 本研究分析了黄花蒿挥发油的化学成分及其对赤拟谷盗成虫和幼虫两个阶段的防治作用。采用水蒸气蒸馏法提取挥发油，GC-MS分析挥发油的化学成分和相对含量。结果表明，黄花蒿挥发油的主要成分和含量为：蒿酮13.2%，β-芹子烯12.3%，樟脑12.2%，桉叶油醇10.3%等。生物活性测试结果显示，黄花蒿挥发油对赤拟谷盗成虫和幼虫的触杀活性LD50分别为37.32 μg/头和70.34 μg/头。同时，黄花蒿挥发油对赤拟谷盗成虫和幼虫均具有明显的驱避作用。因此，本研究能够为黄花蒿挥发油防治粮食仓储害虫提供参考。 关键词 黄花蒿 赤拟谷盗 挥发油 杀虫活性 中图分类号：S433.5 文献标识码：A 文章编号： Study of Biological Activity of Artemisia annua Essential Oil against Adults and Larvae of Tribolium castaneum Cheng Fang Shao Yazhou Yang Yingying Liu Xinying Wang Jingjing Jia Lingyun Liang Junyu * (College of Life Sciences, Northwest Normal University, Lanzhou 730070, China) Abstract In this study, the chemical constituents of Artemisia annua essential oil and its prevention and control effects against T. castaneum adults and larvae were analysed. Essential oil extracted from A. annua by steam distillation method, analysed its chemical composition and relative content through GC-MS. The results showed that the main components of the essential oil are artemisia ketonc (13.2%), β- selinene (12.3%), camphor (12.2%), and eudesmol (10.3%). The results of the bioassay displayed that the contact activity of essential oil from A. annua against T. castaneum adults and larvae with LD50 values of 37.32 μg/adult and 70.34 μg/larva. Meanwhile, the essential oil of A. annua also has an obvious repellent effect against T. castaneum adults and larvae. Therefore, this study can provide a reference for controlling storage pests of essential oil from A. annua. Key words Artemisia annua, Tribolium castaneum, essential oil, insecticidal activity 赤拟谷盗 Tribolium castaneum属 鞘翅目拟步甲科，又称拟 谷盗，是一种遍及全世界的重要储粮害虫，涉及谷物、油料、中药材及动物性产品等。它食性广，寿命长，适应性强，繁殖迅速[1,2]。赤拟谷盗主要为害面粉，其有臭腺分泌臭液，使小麦粉等发生霉腥味（主要使面粉水分上升，黏度、蛋白含量下降），分泌物还包含致癌物苯醌[3]。幼虫蛀食贮存期的烟叶和