大豆脂肪氧化酶—Ⅲ酶联免疫检测试剂盒的制备及其稳定性研究.docxVIP

大豆脂肪氧化酶—Ⅲ酶联免疫检测试剂盒的制备及其稳定性研究 Abstract：In order to realize the rapid screening of soybean material which lacking lipoxygenase-Ⅲ， an ELISA kit was made by double antibody sandwich method which utilizing the polyclonal antibodies and monoclonal antibodies specific for soybean lipoxygenase-Ⅲ. The results showed that the effective time of the ELISA kit was six months， which stored at 2～8℃， and its stability and accuracy were good， it can be used for quick detecting for soybean material which lack lipoxygenase-Ⅲ. Key words：soybean； lipoxygenase-Ⅲ； monoclonal antibody； polyclonal antibody； EL