可口革囊星虫盐度胁迫体腔液.docxVIP

PAGE III PAGE III 摘 要 为了探讨可口革囊星虫(Phasolosma esculenta)耐受低盐下的反应机制, 对照组为自然海水, 研究在低盐度胁迫下星虫的致死率，及胁迫0h、3h、6h、12h、24h和48h时体腔液的Na+、K+、Cl-的浓度和肠、体壁组织的生理指标、组织学的变化。结果表明，随着盐度从10ppt下降到5ppt，可口革囊星虫的致死率逐渐升高。在盐度10ppt作用下，星虫血液的Na+、K+和Cl-的浓度快速下降，而后维持在一定值。肠壁和体壁Na+/K+-ATP酶（NKP）和酸性磷酸酶（ACP）活性分别在6h和12h达到高峰，而后稳定在略高于对照组的水平。在盐分胁迫下，肠和体壁的结缔组织细胞变大而疏松。 研究表明，低盐胁迫下酶系统和非酶系统发生了变化，为揭示低盐胁迫反应的机理提供了部分的线索。 关键词：可口革囊星虫；盐度；胁迫；体腔液  Abstract In order to investigate the response mechanism of Phasolosma esculenta to low salinity, the control group was natural seawater. The mortality rate of Phasolosma esculenta under low salinity stress was studied, as well as the concentration of Na+, K+ and Cl- in the body cavity fluid and the physiological and histological changes of intestinal and body wall tissues under 0h, 3h, 6h, 12h, 24h and 48h stress. The results showed that with the decrease of salinity from 10ppt to 5ppt, the mortality rate of dendrosaceae was gradually increased. Under the action of a salinity of 10ppt, the concentrations of Na+, K+ and Cl- in starworm blood decreased rapidly and then remained at a certain value. The activity of Na+/K+ -atpase (NKP) and ACP in intestinal wall and body wall reached a peak at 6h and 12h respectively, and then stabilized at a level slightly higher than that in the control group. Under salt stress, the connective tissue cells of the intestine and body walls become large and loose. The results showed that the enzyme system and non-enzyme system changed under low salt stress, which provided some clues to reveal the mechanism of reaction under low salt stress. Keywords： Phasolosma esculenta；salinity；stress；coelomic fluid PAGE PAGE IV 目录TOC \o 1-3 \h \u 1 引 言 1 1.1 盐度对可口革囊星虫存活的影响 1 1.2盐度胁迫对渗透压调节机制的影响 1 1.3盐度对免疫力指标的影响 1 1.4 盐度胁迫对组织变化的影响 2 1.5可口革囊星虫简介与研究方向 2 1.6研究目的及主要内容 3 2 材料方法 3 2.1 材料、药品及仪器 3 2.1.1 试验原料 3 2.1.2 仪器 3 2.1.3 药品 3 2.2 方法 4 2.2.1盐度耐受性 4 2.2.2实验处理及采样 4 2.2.3体重变化 4 2.2.4体腔液离子含量 5 2.2.5酶活度测定