基因工程大实验.pptxVIP

基因工程及分子生物学大实验;一、实 验 目 的;二、教 学 组 织;三、实 验 内 容;1. 目 的 基 因;;2. 载 体;pMD18/19-T;pUCm-T;pET-Trx 3.3kb;pGEX-4T1;四、 实 验 流 程;参考计划;实验一 质粒DNA的小量制备 ;;;实验三 质粒DNA的酶切;实验四 PCR扩增制备目的基因;2. PCR仪的循环程序设置：;3. PCR产物电泳;;实验六 目的基因片段与载体连接;实验七 感受态菌的制备;实验八 细菌转化;;电泳鉴定结果;实验十 外源基因的诱导表达;实验十一 SDS检测表达蛋白;1、Genius only means hard-working all ones life. (Mendeleyer, Russian Chemist)? 天才只意味着终身不懈的努力。20.8.58.5.202011:0311:03:10Aug-2011:03 2、Our destiny offers not only the cup of despair, but the chalice of opportunity. (Richard Nixon, American President )命运给予我们的不是失望之酒，而是机会之杯。二〇二〇年八月五日2020年8月5日星期三 3、Patience is bitter, but its fruit is sweet. (Jean Jacques Rousseau , French thinker)忍耐是痛苦的，但它的果实是甜蜜的。11:038.5.202011:038.5.202011:0311:03:108.5.202011:038.5.2020 4、All that you do, do with your might; things done by halves are never done right. R.H. Stoddard, American poet做一切事都应尽力而为，半途而废永远不行8.5.20208.5.202011:0311:0311:03:1011:03:10 5、You have to believe in yourself. Thats the secret of success. Charles Chaplin人必须相信自己，这是成功的秘诀。-Wednesday, August 5, 2020August 20Wednesday, August 5, 20208/5/2020 6、Almost any situationgood or badis affected by the attitude we bring to. Lucius Annaus Seneca差不多任何一种处境无论是好是坏都受到我们对待处境态度的影响。11时3分11时3分5-Aug-208.5.2020 7、Although the world is full of suffering, it is full also of the overcoming of it.Hellen Keller, American writer虽然世界多苦难，但是苦难总是能战胜的。20.8.520.8.520.8.5。2020年8月5日星期三二〇二〇年八月五日 8、For man is man and master of his fate.Tennyson人就是人，是自己命运的主人11:0311:03:108.5.2020Wednesday, August 5, 2020 9、When success comes in the door, it seems, love often goes out the window.Joyce Brothers成功来到门前时，爱情往往就走出了窗外。11:038.5.202011:038.5.202011:0311:03:108.5.202011:038.5.2020 10、Life is measured by thought and action, not by time. ——Lubbock 衡量生命的尺度是思想和行为，而不是时间。8.5.20208.5.202011:0311:0311:03:1011:03:10 11、To make a lasting marriage we have to overcome self-centeredness.要使婚姻长久，就需克服自我中心意识。Wednesday, August 5, 2020August 20Wednesday, August 5, 20208/5/2020 12、Treat other people as you hope they