第六章色谱分析导论.ppt

Chapter 6 An introduction to chromatographic separation Outline General information Special terms Basic principles Separation, band broadening and efficiencies Applications Quantitative and qualitative Chromatography – “color” and “to write” Originally described by Tswett – 1906 He devised a method to separate plant pigments using a tube filled with CaCO3. After adding a plant extract, he was able to produce several colored bands by washing the extract through the column with an organic solvent. The separation of a mixture by distribution of its components between a mobile and stat