CD157在PNH诊断中的应用 ppt课件.pptx

CD157鉴定粒/单核细胞PNH克隆;概 要;精品资料; 你怎么称呼老师？ 如果老师最后没有总结一节课的重点的难点，你是否会认为老师的教学方法需要改进？ 你所经历的课堂，是讲座式还是讨论式？ 教师的教鞭 “不怕太阳晒，也不怕那风雨狂，只怕先生骂我笨，没有学问无颜见爹娘 ……” “太阳当空照，花儿对我笑，小鸟说早早早……”;What is PNH;;European Journal of Haematology, 2015;Consequences of Hemolysis in PNH;Pathogenesis of PNH;Classification scheme for PNH;Diagnosis of PNH;The Problem With CD55/CD59;Results of initial EQA study;14;15;Detecting 1% clone sizes Adequate for patients with large clones associated with hemolytic/thrombotic PNH Testing only red cells may miss patients Possible to test both monocytes and granulocytes as one serves as a check for the other Lymphocytes not a suitable target;High sensitivity assays;Prevalence;High sensitivity assays;How many cells to collect;Routine RBC assays;Reagents used in PNH analysis;WHAT IS FLAER?;需进行PNH检测临床体征;随访检测;流式细胞术检测PNH;;;;;;;;Possible WBC reagent combinations;How does Flear Work?;Summary and conclusions flow cytometry in PNH;Questions;38;;Cytometry Part B (Clinical Cytometry) 86B:44–55 (2014);A single-tube/5-color, CD157-based assay confirmed very good performance characteristics for both intra- and interassay precision, excellent correlation, and agreement between approaches, which supports CD157 for a rapid and cost-effective evaluation of PNH by flow cytometry.;本课题拟解决的问题;材料和方法;结果;PNH阴性标本;PNH阳性微小克隆;PNH阳性克隆;CD157异常证实可疑PNH克隆;7例标本粒/单核细胞表面CD157表达同时缺失;讨论;谢 谢！