耻垢分支杆菌基因敲除交流.pptVIP

* * * 耻垢分支杆菌基因敲除交流 内容 sacB 作为负筛标记 sacB , which encodes the Bacillus subtilis secreted enzyme levansucrase Levansucrase catalyzes hydrolysis of surose and synthesis of levans , which are high-molecular-weight fructose polymers Expression of sacB is lethal to Mycobacterium smegmatis in the presence of 10% sucrose The molecular basis of the toxicity is still unclear Pelicic V, Reyrat JM, Gicquel B. Expression of the Bacillus subtilis sacB gene confers sucrose sensitivity on mycobacteria. J Bacteriol 1996;178:1197-9. 构建自杀质粒 1165 bp 6359 bp 5409 bp pMind-2630UD-sacB digests with Pac I and Spe I