四种测定纤维素酶发酵过程生物量方法的比较.docxVIP

四种测定纤维素酶发酵过程生物量方法的比较 四种测定纤维素酶发酵过程生物量方法的比较 四种测定纤维素酶发酵过程生物量方法的比较 李晨1，2?赫荣琳2?武改红2?马立娟2?路福平1?陈树林2 （1.天津科技大学生物工程学院，天津 300457；2.中国科学院天津工业生物技术研究所 天津市工业生物系统与 过程工程重点实验室，天津 300308） 摘 要： 分别采用测定菌丝体蛋白的方法、测定核酸的方法、酸洗干燥法和纤维素测定仪的方法测定以不溶性纤维素为底物发酵产纤维素酶过程中的生物量。通过对发酵液取样测定比较，结果发现，采用测定核酸的方法精密度最高，其RSD值为6.32%，该法同纤维素测定仪的方法均可用于精确测定以不溶性纤维素为底物发酵产纤维素酶过程中的生物量。酸洗干燥法的测定结果精密度较差一些，但由于操作简便，也可用于常规分析。而测定菌丝体蛋白的方法无论是在精密度上还是操作简便性上均不占优势，该方法可作为另外3种方法的补充。为以不溶性纤维素为底物发酵产纤维素酶过程中生物量的分析检测提供了选择方法的依据。 关键词： 纤维素 纤维素酶 生物量 菌丝体蛋白 核酸 酸洗 纤维素测定仪 Comparison of Four Methods for Measuring the Biomass of the Cellulase Production Process （1College of Bioengineering， Tianjin University of Science Technology， Tianjin 300457；Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering， Tianjin Institute of Industrial Biotechnology， Chinese Academy of Sciences， Tianjin 300308） Li Chen1， He Ronglin2 Wu Gaihong2 Ma Lijuan2 Lu Fuping1 Chen Shulin2 Abstract:?Biomass of the cellulase production process with insoluble cellulose as the substrate was measured using mycelial protein determination, nucleic acid determination, acid washing and drying method as well as cellulose analyzer application, respectively. Measurement results of fermentation broth samples indicated that the precision of nucleic acid determination method was the highest with RSD value at 6.32%. This method and cellulose analyzer application method could be used to determine the biomass of the cellulase production process with insoluble cellulose as the substrate more accurately. The precision of acid washing and drying method was poorer, but it could be used for routine analysis of biomass due to its simple operation. The mycelial protein determination method was proved to be poorer neither in precision nor in operation simplicity, and it could be used as the complement of the other three methods. This study provides a basis for selecting method in measuring the biomass of the cellulase produc