核分析法与生命科学中的微量元素：第四章 PIXE.pptx

第四章 PIXE;PIXE原理;本底来源;本底来源;PIXE分析灵敏度;PIXE分析灵敏度;定量分析 ;X射线分析谱仪;束流测量;Software developement;不同激发源对样品的损伤比较;实验考虑;multi-elemental？;multi-elemental？;样品制备;Untreated bone samples;Sample preparation;Quantitative PIXE Measurements;Quantitative PIXE Measurements; The dried bone was ground into a very fine powder uniformity A hydraulic press capable of creating high and reproducible pressure was used to compress the bone powder to a self-supporting specimen flat and smooth;K.Ishii, et al, Physics Review A15(1977)2126 ;F.Folkmann, Nuclear Instruments and Methods in Physics Research B3 (1984) 11 ;Measured X-ray spectra at 90°, 135”, and 160’;J.L.Campbell, Nuclear Instruments and Methods in Physics Research B3 (1984) 185;Absorption filter;吸收片;In order to properly apply the PIXE method using a funny filter, a very precise characterization of the analytical system geometry is required;New sample holder;Non-destructive (damage)?;Concentration of one treated bone sample detected by 7 times ;Micro-PIXE line-scan;Otoliths’ cores are not in the geometric center; Ca map of the otolith and the selected raster scan of Sr map were measured. The raster scan pattern is showed with real lines and the high Sr region is showed with dash lines. ;;;Two-dimensional analysis of a whole single cell;;Euglena cell;Accuracy and precision of around 5% is attainable using PIXE Precautions for accurate quantitative analysis sample preparation experimental setup radiation damage normalization data processing …;”Total reflection”;Depth-resolved PIXE-studies;PIXE分析的特点;Applications-anything from …;Over….;To….;TERMITE;The total amount of the 14 predominant elements in an intact termite;Among worker samples;;; 稀土在细胞内的分布和作用 ;大鼠肝细胞; ;The influence of REEs on the Ca2+ content in rat hepatocyte;稀土元素La倾向于积累在肝脏中（灌胃稀土浓度为0.02mg/kg）;高浓度时肝内稀土浓度反而降低;PIXE determination of Pb in wine ;Pb Lα X-ray intensity versus the amount of Pb added to the white wine SCh ;Dependence