小儿哮喘与树突状细胞免疫分子表达异常关系研究.pdfVIP

小儿哮喘与树突状细胞免疫分子表达异常关系研究 目的 比较哮喘患儿与正常 DCs 表达协同刺激分子(B7-1 和 B7-2)和主要组织 相容性复合体分子(MHC Ⅰ和Ⅱ类分子)的水平，研究小儿哮喘的免疫学发生机制。 方法 分离 DCs，采用酶免疫标记技术分别检测并比较各组 DCs 协同刺激分子 （B7-1 和 B7-2）、主要组织相容性复合体分子（MHC Ⅰ和Ⅱ类分子）表达率。结 果 DCs 细胞表面 B7-1 表达率在哮喘组高于对照组，分别为 26.02±7.26%和 18.17±5.21%，差异具有显著性（p0.05）；而哮喘组 DCs 表面 B7-2 表达率低 于对照组分别为 9.22±2.15%和 16.18±3.81%，差异具有显著性(p0.05)。 DCs 表面 MHC- Ⅰ分子表达率在小儿哮喘组和对照组分别为52.02±12.18%和 58.62±9.26%，差异不具有显著性（p0.05）；DCs 表面 MHC- Ⅱ表达率在小儿 哮喘组和对照组分别为 56.26±8.37%和 61.08±11.95%，差异不具有显著性 (p0.05)。结论 小儿哮喘的免疫学发生机制与 DCs 协同刺激分子表达功能异常 密切相关。 关键词 小儿哮喘，DCs，协同刺激分子， 主要组织相容性复合体 Study on the Relationship Between Asthma in children and the Immuno-molecular Abnormal Expressions of DCs ABSTRACT Objective By comparing the different expressions of immuno- moleculars of DCs between asthma in children and control, so to study the immune mechanism of asthma in children. Methods DCs were isolated from all the surveys, immune lable technique were performed to detected the expressioons of co-stimulatory molecules and MHC molecules on the surface of DCs. Results The expression of B7-1 on DCs from asthma in children was higher than that from control group, they were 26.02±7.26% and 18.17±5.21% respectively, while the expression of B7-2 was seen a reverse tendency, 9.22±2.15% and 16.18±3.81% ， respectively. The expression rates of MHC I molecule on the surface of DCs from asthma in children and control group were 52.02±12.18% and 58.62±9.26% respectively, and the difference has no statistical meaning. About MHC Ⅱmolecule, it was seen the same result between the 2 groups, and the expression rates of asthma in children and control group were 56.26±8.37% and 61.08±11.95% respectively. Conclusions The immune mechanism of asthma in children was related t