资料分享在存在新城疫母源性抗体下,嵌合新城疫病毒能抵抗禽流感病毒.pptxVIP

Chimeric Newcastle Disease Virus Protects Chickensagainst Avian Influenza in the Presence of MaternallyDerived NDV ImmunityConstruction, transfection and recovery of chimeric NDV321chNDVFHN PMV8H5 replicates efficientlyDetermination of virulenceExpression and virion incorporation of the foreign proteins of chNDVFHNPMV8H5chNDVFHN PMV8H5 replicates efficientlychNDVFHN PMV8H5 replicates efficientlychNDVFHN PMV8H5 was passaged ten times on ECE,resulting in virus stocks with comparably high titers (about 108TCID50/ml in 3rd and 10th passage)This demonstrates continuing co-expression of the transgenesand the resident NDV gene indicating a highly stable expression of all genes of the new chimeric virusReplication of chNDVFHN PMV8H5 in chickensTwo naive sentinel animalsReplication of chNDVFHN PMV8H5 in chickensReplication of chNDVFHN PMV8H5 in chickensNone of the inoculated animals showed any clinical signshistological examination post necropsy of two chickens each on 2, 4, 6 and 20 dpvno lesions or inflammation any of the tissues investigatedonly a local infection in the upper respiratory tract.chNDVFHN PMV8H5 protects chickens againstlethal HPAIV infectionchNDVFHN PMV8H5 protects chickens againstlethal HPAIV infectionNDVH5VmchNDVFHN PMV8H5 protects chickens againstlethal HPAIV infectionchNDVFHN PMV8H5chNDVFHN PMV8H5 protects chickens againstlethal HPAIV infectionchNDVFHN PMV8H5chNDVFHN PMV8H5 protects chickens againstlethal HPAIV infectionchNDVFHN PMV8H5DiscussionchNDVFHN PMV8H5 is a possible alternative vaccine candidatefor a strategy using two different vaccines in breeders and in offspringA disadvantage of the use of chNDVFHN PMV8H5 in comparison to NDVH5Vm is the loss ofprotection against NDFurther investigations have to demonstrate the impact of this vaccine on reducing spread of HPAIV H5 in AIV vaccinated populationsThankyou