mir-17通过靶向胰岛素样生长因子1调节冠状动脉疾病中血管平滑肌细胞增殖.docxVIP

第 第42卷第4期 2 0 2 1年7月 西安交通大学学报(医学版) Journal of Xian Jiaotong University (Medical Sciences) ?基础研究? miR-17通过靶向胰岛素样生长因子1调节冠状动脉疾病中血管平滑肌细胞增殖 金爱萍，李书琳，张倩榕，李冰 (西安交通大学第二附属医院老年心血管科，陕西西安710004) 摘要：可的 研究miR-17对冠状动脉疾病(CAD)中血管平滑肌细胞(VSMCs)的潜在作用机制。方法 RT-qPCR检 测CAD患者血清和VSMCs中miR-17和胰岛素样生长因子l(IGF-l)的mRNA表达，通过CCK-8和克隆形成实验 测定VSMCs的增殖情况；通过生物信息学和荧光素酶报告基因检测系统分析miR-17的潜在靶标。结果 RT-qPCR 结果显示，与对照组相比，CAD患者血清和VSMCs中miR-17的mRNA表达均显著上调(PV0.01)。CCK-8和克隆 形成测定结果显示，与对照组相比,miR-17过表达组VSMCs细胞增殖和克隆形成数均显著升高(PV0. 01)； miR-17 低表达组均显著降低(FV0.01)。生物信息学分析结果显示，IGF-1的3-UTR中具有miR-17的结合位点。荧光素 酶报告基因测定结果显示，与野生型IGF-1质粒和miR-17模拟物共转染的VSMCs的荧光素酶活性升高(P0. 001), 而转染突变型IGF-1质粒和miR-17模拟物的VSMCs的荧光素酶活性无改变。与对照组相比，过表达miR-17组 VSMCs中的IGF-1表达上调(P0.01)；与对照组相比JGF-1过表达组VSMCs细胞增殖和克隆形成数均显著升高 (P0.05)o结论 miR-17通过靶向IGF-1促进VSMCs增殖，表明miR-17可作为CAD的预测生物标志物JGF-1 可能是潜在的治疗靶点。 关键词：冠状动脉疾病；miR-17；胰岛素样生长因子1;细胞增殖 中图分类号:R543.3 文献标志码：A DOI： 10. 7652/jdyxb202104002 miR-17 targets IGF-1 to regulate vascular smooth muscle cellproliferation in coronary artery disease JIN Aiping, LI Shulin, ZHANG Qianrong, LI Bing (Department of Elderly Cardiovascular Medicine, The Second AffiliatedHospital of Xian Jiaotong University, Xian 710004, China) ABSTRACT: Objective To investigate the potential mechanism of miR-17 in vascular smooth muscle cells in coronary artery disease (CAD). Methods mRNA expression of miR-17 and insulin growth factor 1 (IGF-1) in serum and VSMCs of CAD patients were detected by RT?qPCR. Potential targets of miR-17 were detected by bioinformatics and luciferase reporter assay； CCK-8 and cloning formation assay was performed to measure the proliferation of VSMCs. Results RT-qPCR results showed that compared with those in control group, the miR-17 mRNA expression in VSMCs and serum of CAD patients were significantly upregulated (PV0.01). 1 he results of CCK-8 and clone formation assay showed that compared with those in control group, the number of VSMCs proliferation and cloning formation in the miR-17 overexpression group were significantly increa