MakingSingleMoleculeFluorescenceLifetimeMeasurementsSimple课件讲解英文.pptxVIP

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MakingSingleMoleculeFluorescenceLifetimeMeasurementsSimple课件讲解英文.pptx

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Making Single Molecule Fluorescence Lifetime Measurements Simple;Joint workshop between:;SCATTERING;Jablonski Diagram;Fluorescence is an Exponential Decay Process;Parameters that affect Fluorescence 1- Pressure 2- Temperature 3- Electrical Potential 4- ions 5- viscosity 6- pH 7- ...;Fluorophores ?;Fluorophores - 2;J6rg Enderlein (Forschungszentrum JUlich, Germany). Single Molecule Fluorescence Spectroscopy9;Ted Laurence (Lawrence Livermore National Laboratory」Livermore, USA). Probing Structural Heterogeneities and Fluctuations of Nucleic Acids and Denatured Proteins using Single-Molecule Fluorescence Lifetime Spectroscopy9 Goals : 1- Reach Accurate FRET Measurements (1ns to 100 microsec), see next picture 2- Polarization Effects on Fluorophores Applications : ? Evaluate distance between a DONOR Molecule and a Acceptor Molecule in order to characterize unfolded proteins and the measured lifetimes (Residual Photon Counts);Fluorescence Resonance Energy Transfer;Abigail Miller (UC Berkeley, USA). Fluorescence Correlation Spectroscopy of a Novel Genetically Encodable Red-Emitting Fluorescent Protein;Samantha Fore (UC Davis, Sacramento, USA). Applications of Photon Antibunching in Biology;Rainer Erdmann (PicoQuant GmbH, Germany). The MicroTime 200 ■ An All In One Solution For Time- Resolved Confocal Microscopy;Markus Jager (UCLA USA). Probing early events in protein folding by single molecule FRET and microfluidic laminar flow mixing;Daniele Gerion (Lawrence Livermore National Laboratory, Livermore, USA). Qdot~based Probes for Watching Biomolecules Rockn Roll;Uwe Ortmann (PicoQuant GmbH Germany). FLIM and FCS Upgrade Kit for Laser Scanning Microscopes n;Thomas Dertinger (Forschungszentrum JUlich. G eri , uTwo Focus FCS using PIE;Michael B6rsch (University of Stuttgart Germany). Three-Dimensional Localization of the a-Subunit In F0F1-ATP Synthase by Time Resolved Single-Molecule FRETn;Standard Fluorescence Spectrometer;Photon Counting Instrumentation ■ 1;Photon Counting