API3000LCMSMS操作培训手册.pptxVIP

API 3000 Operator Training Course Analyst-NTFoster City CA PAL LabWhat is LC/MSLC: Liquid Chromatography MS: Mass Spectrometer an instrument that produces ions and separates them in the gas phase according to their mass-to-charge ratio (m/z)Element MassAverage mass of all natural isotopesCarbon Abundance Integer Mass12C = 12.0000 Da 98.91% 1213C = 13.0034 1.09% 13(12.0000)(0.9891) + (13.0034)(.011) = 12.011Natural isotopes AbundanceElement Abundance Exact Mass X+1 Factor X+2Factor 1H 99.99 1.0078 2H 0.01 2.0141 10B 49.9 10