核酸的生物合成和降解.ppt

核酸的生物合成和降解;一. DNA复制;(一) 复制反应;(二) 复制方法;半保留复制;怎样证实半保留复制;(二) 复制方法;(三) 复制反应注意点;DNA聚合酶;More than 90 % of the DNA polymerase activity observed in E. coli extracts can be accounted for by DNA polymerase I ;(四)参与复制酶和蛋白质;(四)参与复制酶和蛋白质;DNA聚合酶;(四)参与复制酶和蛋白质;3→5核酸外切; Every cell contains several different nucleases (核酸酶), belonging to two broad classes: exonucleases (核酸外切酶)and endonucleases (核酸内切酶). Exonucleases degrade nucleic acids from one end of the molecule. 核酸外切Exonucleases degrade nucleic acids from one end of the molecule. Many operate in only the 5 → 3 or the 3→5 direction, removing nucleotides on