分子印迹渍与分子杂交技术.pptVIP

转膜 Protein HRP DAB H2O2 1Ab 2Ab 洗膜、显色 第三十页，共59页 mRNA = messenger RNAECD = extracellular domainELISA = enzyme-linked immunosorbent assayIHC = immunohistochemistryCISH = chromogenic in situ hybridizationPCR = polymerase chain reactionFISH = fluorescence in situ hybridization 第三十一页，共59页 1.Extract DNA from cell Extract RNA from cell Extract protein from cell Southern blot Northern blot Western blot 2.Cut with restriction enzyme 3.Run on gel(usually agarose) Run on gel(usually agarose) Run on gel(usually poly -