杜仲饮片HPCE指纹图谱研究(中药材)讲述.docVIP

杜仲饮片HPCE指纹图谱的研究(中药材)讲述 杜仲饮片HPCE指纹图谱的研究(中药材)讲述 杜仲饮片HPCE指纹图谱的研究(中药材)讲述 杜仲饮片 HPCE 指纹图谱的研究 摘要 目的： 建立杜仲饮片 HPCE-DAD 指纹图谱分析方法，并对杜仲及其炮制品的指纹谱进行比 较。方法： 采用高效毛细管电泳法进行色谱分离，以 60mmol·L –1 硼砂 -20 mmol·L –1 磷酸二氢钠 -10% 甲醇 (pH= 10.0) 为运行缓冲液，分离电压为 20 kV ，波长为 210nm，以松脂醇二葡萄糖苷为参照物（ IS），测定 其指纹图谱，并作模糊聚类法分析和相似度评价。结果： 初步建立了以 10 个共有峰为特征指纹信息的杜 仲饮片 HPCE-DAD 指纹图谱； 发现少数杜仲饮片的指纹图谱有一定差异， 生品与其炮制品的指纹谱中共有 峰相对峰面积差异显著。结论： 方法准确可靠，重现性好，可作为杜仲饮片内在质量评价的依据。 关键词 杜仲； 高效毛细管电泳二极管阵列检测法 ；指纹图谱 Studies on HPCE-DAD fingerprint of Eucommiae Cortex FU Xing-sheng, HAN Le , LIU Xun-hong*, XU Hu , LI Jun-song , CAI Bao-chang ， YANG Yan (Nanjing University of Chinese Medicine, Nangjing 210046, China) Abstract Objective: To establish the analytical method for the fingerprint of Eucommiae Cortex by HPCE-DAD and compare the fingerprints of Eucommiae Cortex and its processed products. Method: Based on the mode of high performance capillary electrophoresis， 60mmol·L-1 sodium borate-20 mmol·L –1 sodium dihydrogen phosphate-10% methanol (pH10.0) was used as buffer solution. The separation voltage was 20 kV and the detection wavelength was set at 210 nm. Pinoresinol diglucoside was used as a reference standard，the chromatographic fingerprint were determined. The data were analyzed by fuzzy cluster and fingerprint similarity evaluation software to compare the similarity of samples. Result: HPCE-DAD fingerprints with 10 common peaks of Eucommiae Cortex were established preliminarily. It was discovered that a small number of samples differed from others. Regarding to the fingerprints of Eucommiae Cortex and its processed products，there were obvious differences in the relative areas of common peaks.Conclusion: The method is reliable ， accurate and can be used for quality control of Eucommiae Cortex. Key words Eucommiae Cortex ；HPCE-DAD； Fingerprint 杜仲为杜仲科植物杜仲 Eucommia ulmoides Oliv. 的干燥树皮，具有补肝肾，强筋骨，安 胎等功效，用于肝肾不足、腰膝酸痛、筋骨无力、头晕目眩、妊娠漏血、胎动不安等症 [1] 。 现代研究表明， 松脂醇二葡萄糖苷、 绿原酸和环烯醚萜苷类为主要活性成分。目前，多采用 高效液相色谱法， 以松脂醇二葡萄糖苷或绿原酸等含量为质控指标， 分析和评价杜仲药材或