干细胞系质量控制程序.pdfVIP

CHAPTER 1 QUALITY CONTROL PROCEDURES FOR STEM CELL LINES 1 2 GLYN N. STACEY and JONATHAN M. AUERBACH 1 Division of Cell Biology and Imaging and the UK Stem Cell Bank, National Institute for Biological Standards and Control, South Mimms, Herts., EN6 3QG, UK 2 gstacey@nibsc.ac.uk; GlobalStem, Inc., Rockville, MD 20850, USA, JAuerbach@GlobalS 1.1. Introduction 2 1.2. The Cell Banking Principle 3 1.3. Cell Characterization 5 1.3.1. Viability 5 1.3.2. Karyology 5 1.3.3. Identity Testing 7 . Con?rmation of Species of Origin 7 . DNA Pro?ling for Cell-Speci?c Identi?cation 8 . Antibody Markers 10 . Gene Expression 11 . Pluripotency 12 1.4. Sterility 12 1.5. Mycoplasma Testing 14 1.6. Other Microbial Contaminants and Potential Biohazards 14 1.7. Quality Control of Culture Conditions, Reagents, and Media 17 1.8. Conclusions 17 References 17 Appendix 1. Some Point