biochemistry昆虫细胞系转录组.pdfVIP

Insect Biochemistry and Molecular Biology 59 (2015) 1 17 Contents lists available at ScienceDirect Insect Biochemistry and Molecular Biology journal homepage: Endogenous expression of a Bt toxin receptor in the Cry1Ac-susceptible insect cell line and its synergistic effect with cadherin on cytotoxicity of activated Cry1Ac a, 1 a, 1 b b a a Zuwen Chen , Fei He , Yutao Xiao , Chenxi Liu , Jianghuai Li , Yongbo Yang , Hui Ai a, Jianxin Peng a, Huazhu Hong a, Kaiyu Liu a, * a School of Life Sciences, Central Normal University, Wuhan 430079, PR b State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant , Academy of Agricultural Sciences, 100193, PR a r t i c l e i n f o a b s t r a c t Article history: Although many insect cell lines derived from various tissues are available, it is unclear whether Received 25 October 2014 endogenous receptors of Bacillus thuringiensis (Bt) crystal toxins are expressed in these cell lines. In the Received in revised form present study, we demonstrated that the ovaries derived Spodoptera litura Sl HP cell line was susceptible 18 January 2015 to activated Cry1Ac although larvae of S. litura are not susceptible to the toxin. Assays of the Accepted 23 January 2015 transcriptome revealed that thirteen ATP bind