新型螯合剂对镉致小鼠睾丸毒性的解毒作用.docVIP

新型螯合剂对镉致小鼠睾丸毒性的解毒作用 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：新型螯合剂对镉致小鼠睾丸毒性的解毒作用 1 1 材料与方法 3 16 统计分析 采用SPSS90统计软件进行t检验。 4 2 结果 4 3 讨论 6 文2：镉对小鼠睾丸精子生成的影响及其分子机制 6 1 材料和方法 7 2 结果 9 3 讨论 9 参考文摘引言： 11 原创性声明（模板） 12 文章致谢（模板） 12 正文 新型螯合剂对镉致小鼠睾丸毒性的解毒作用 文1：新型螯合剂对镉致小鼠睾丸毒性的解毒作用 Effects of new chelating agents on testicular toxicity in mice induced by acute exposure to cadmium Abstract： Objective To study the effects of new chelating agents N-p-hydroxy-methyl-benzyl-D-glucamine dithiocarbamate(HBGD)and N-benzyl-D-glucamine dithiocarbamate(BGD) on testicular toxicity in mice induced by acute exposure to HBGD，BGD，diethyldithiocarbamate(DDTC)，2，3-dimercaptopropanol(BAL)，and ethylene diaminetetraacetic acid(EDTA) were studied for their effects on the concentratio of cadmium(Cd)，calcium(Ca)，iron(Fe)，and zinc(Zn) in testes，lipid peroxidation(LPO) value of testes，activity of lacatate dehydrogenase(LDH) in serum and reproductive ability of male mice induced by acute exposure to Mice were injected intraperitoneally with CdCl2(/kg) and after 30min or 24h，they were injected intraperitoneally with chelating agents(400μmol/kg).Cd injection increased in the concentratio of Ca，Cd，Fe，Zn，and LPO value in testes as well as the activity of LDH in serum of and BGD effectively prevented the increase in above indices，and clearly improved the reproductive ability weakened by exposure to HBGD and BGD are not only more effective detoxificationon testicular toxicity in mice induced by acute exposure to Cd，but also less toxic，so they are pretect antidote for Cd. Key words： cadmium;chelating agents;testicular toxicity；detoxification 镉是一种对生物体多种脏器都有毒性的有毒重金属〔1-3〕，被机体吸收后， 自然 排泄非常缓慢，因此，用解毒剂驱排体内蓄积的镉是防治镉中毒的根本措施之一。但目前临床上使用的镉解毒剂由于毒副作用较大，使用效果不理想。本研究通过比较新合成的2种螯合剂N-对羟甲苯甲基-D-葡萄糖二硫代氨基甲酸钠(HBGD)与N-苯甲基-D-葡萄糖二硫代氨基甲酸钠(BGD)及现有螯合剂二乙基二硫代氨基甲酸钠(DDTC)、二巯基丙醇(BAL)及乙二胺四乙酸(EDTA)对镉致小鼠睾丸毒性的解毒作用，为开发解镉中毒的高效低毒的新型解毒剂提供依据。 1 材料与方法 11 仪器与试剂 Z-8000型原子吸收分光光度计(日本日立公司)；UV-210型分光光度计(日本岛津公司)；Optima TL型冷冻离心机(美