非小细胞肺癌转化生长因子β的表达及其与血管生成的关系.doc

非小细胞肺癌转化生长因子β的表达及其与血管生成的关系 作者：卫小红，杜凤兰，王鸿雁 【关键词】 非小细胞肺癌 　　【Abstract】 AIM: To investigate the correlation between the expression of transforming growth factor beta (TGFβ) and the angiogenesis in nonsmall cell lung cancer (NSCLC). METHODS: MVC and expression of TGFβ1 in 50 carcinomatous specimens and expression of TGFβ1 in 35 paracarcinomatous specimens from the same 50 cases with NSCLC were detected by SP immunohistochemistry. RESULTS: Active angiogenesis in NSCLC was detected and it was related with NSCLC progression and lymph node metastasis, but not related with histological types. Significant difference of the expression of TGFβ1 was found between stage Ⅰ and Ⅱ (Plt;0.05), and between stage Ⅰ and Ⅲ (Plt;0.05), and the positive expression of TGFβ1 was significantly higher in the patients with lymph node metastasis than those without lymph node metastasis (Plt;0.01). No significant association was found between the TGFβ1 expression and the characteristics of the histological types in NSCLC. Positive TGFβ1 expression in carcinomatous specimen was stronger than that in paracarcinomatous specimen. TGFβ1 expression was correlated with tumor angiogenesis. CONCLUSION: TGFβ highly promotes the progression and lymph node metastasis of NSCLC, mainly by stimulating angiogenesis. 　　【Keywords】 NSCLC; TGFβ; angiogenesis; immunohistochemistry 　　【摘要】 目的：研究血管生成与转化生长因子β（TGFβ）在非小细胞肺癌（NSCLC）的表达及其相互关系. 方法：通过应用SP免疫组化方法检测50例NSCLC组织内微血管计数（MVC）及50例NSCLC组织和35例癌旁组织中TGFβ1的表达. 结果：NSCLC癌组织中存在活跃的血管生成，血管生成与病变分期进展和淋巴结转移有关，与组织分型无关. TGFβ1阳性表达在NSCLC的I期与II期及I期与III期之间有显著意义（Plt;0.05），与淋巴结转移有关，与NSCLC组织分型无关；NSCLC中TGFβ1表达与癌旁组织中比较有显著意义（Plt;0.05）；TGFβ1阳性表达与肿瘤血管生成有关. 结论：TGFβ主要通过促肿瘤血管生成而促进了NSCLC的病期进展和淋巴结转移. 　　【关键词】 非小细胞肺癌；转化生长因子β；血管生成；免疫组织化学 　　0引言 　　肿瘤的血管生成（angiogenesis）与肿瘤的发生、发展和转移的全过程密切相关. 肺癌细胞通过合成、分泌血管生成因子调节肿瘤组织的血管生成从而影响肺癌的发生、发展和转移，其中的转化生长因子β(transforming growth factorβ, TGFβ)在原发性肺癌的表达、生物学作用及其与血管生成的关系为人们所关注. 为进一步了解其在非小细胞肺癌（nonsmall cell lung cancer, NSCLC）的进展和转移中的表达及与血管生成的关系，我们采用免疫组化的方法对TGFβ1在非小细胞肺