汉黄芩素对移植人卵巢癌SKOV3细胞裸鼠组织端粒酶蛋白hTERT影响与凋亡相关性研究药学.docVIP

汉黄芩素对移植人卵巢癌SKOV3细胞裸鼠组织端粒酶蛋白hTERT影响与凋亡相关性研究药学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：汉黄芩素对移植人卵巢癌SKOV3细胞裸鼠组织端粒酶蛋白hTERT影响与凋亡相关性研究药学 1 1 材料 3 2 方法 3 3 结果 6 4 结论 7 文2：柴胡皂苷诱导人卵巢癌SKOV3细胞凋亡的作用机制 9 2 结果 13 3 讨论 14 参考文摘引言： 16 原创性声明（模板） 17 文章致谢（模板） 18 正文 汉黄芩素对移植人卵巢癌SKOV3细胞裸鼠组织端粒酶蛋白hTERT影响与凋亡相关性研究药学 文1：汉黄芩素对移植人卵巢癌SKOV3细胞裸鼠组织端粒酶蛋白hTERT影响与凋亡相关性研究药学 Abstract：Objective Regulating telomerase activation is an ideal way to treat cancer . Current studies show that wogonin has the attributes of antioxidation and inhibition on the growth of tumor cells . This study aimed at evaluating the relatiohip between telomerase activation and apoptosis after wogonin administration in nude mice which implanted human ovarian cancer cells SKOV3. MethodsNude mice with implanted human ovarian cancer cells SKOV3 were randomly divided into five groups, including high dose group ,low dose group , normal control group , cisplatin therapy group, and combined therapy group (cisplatin and wogonin) . The expression of hTERT, Bcl-2, Bax and the apoptosis index were detected by The results showed that wogonin could significantly suppress the growth of tumor compared with controlled group. The down expression of telomerase activation and the effectiveness was related to the dose of wogonin . With the increase of suppressing on tumor ,the expression of Bcl-2 decrease regularly , and it showed the opposite tendency of the apoptosis index . While the expression of Bax increased regularly ,and it showed the same tendency with the change of apoptosis expression. ConclusionWogonis can suppress the growth of tumor and regulate telomerase activation. The effectiveness is related to the dose of wogonin , the effect of wogonin would be better if it is combined with cisplatin in the treatment of tumor . It downregulates the activation of telomerase accompanied with apoptosis of cells ,the possible reason is that it damages the structure of telomerase and switch on apoptosis gene . Key wor