离体猪肝胆管腔内射频消融后的胆管和肝脏病理学改变.docVIP

离体猪肝胆管腔内射频消融后的胆管和肝脏病理学改变 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：离体猪肝胆管腔内射频消融后的胆管和肝脏病理学改变 1 1 材料与方法 2 2 结果 3 3 讨论 4 文2：肝门部胆管腔内射频消融相关并发症活体实验观察 5 1　材料与方法 6 2　结果 7 2.４　病理组织学检查结果 8 3　讨论 9 参考文摘引言： 10 原创性声明（模板） 12 文章致谢（模板） 12 正文 离体猪肝胆管腔内射频消融后的胆管和肝脏病理学改变 文1：离体猪肝胆管腔内射频消融后的胆管和肝脏病理学改变 Abstract： Objective To observe the pathological changes of bile duct and liver after radiofrequency ablation (RFA) via the lumen of bile duct and to investigate the sizes of coagulation lesio corresponding to different ablation paramete (length of bared electrode, power output and ablation duration). Methods Fortyeight bile duct targets of liver lobes in 20 isolated porcine live were divided into 4 groups averagely according to different ablation paramete. RFA was performed by ierting a bared electrode into the lumen of bile duct. The sizes of coagulation lesio in section were detected and the pathological changes were observed under microscope. Results Semioval pale coagulation lesio in section, with long axes along bile duct and short axes along the wall of bile duct and hepatic tissue, were observed. Necroses of mucosa and submucosa of bile duct and denaturation of hepatic tissue were found under microscope. The long axial diamete and short axial radii of coagulation lesio varied with different ablation paramete. Conclusion RFA via the lumen of bile duct of liver lobe can produce coagulation lesion. The long axial diameter of coagulation lesion is related to the length of electrode, and the short axial radius related to the power output and the ablation duration. Key words： radiofrequency ablation; lumen of bile duct; pathology; animal experiment 射频消融已广泛应用于 治疗 不能切除的肝癌[1]、肺癌[2]、骨肉瘤[3]、肾肿瘤[4]、乳癌[5]等，但尚未见到经胆管腔内射频消融治疗肝门部胆管癌的报道。本实验观察猪肝叶胆管腔内射频消融后胆管和肝脏病理学变化并比较不同裸露电极针长度、输出功率和消融时间对胆管和肝脏病理学变化范围大小的影响，为射频消融治疗肝门部胆管癌提供依据。 1 材料与方法 实验材料与设备 新鲜离体猪肝20个，重量1 540 g～1 920 g，平均1 730 g。射频发生器（Elektrotom 106 HiTT，Berchtold GmbH andCo，Germany）。裸露电极针长15 mm，直径 mm。根据需要将裸露电极针磨短、磨钝，长度依次