沙漠干热环境下猪腹部肠管火器伤后肝组织中NFκBTNFα的表达临床医学.docVIP

沙漠干热环境下猪腹部肠管火器伤后肝组织中NFκBTNFα的表达临床医学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：沙漠干热环境下猪腹部肠管火器伤后肝组织中NFκBTNFα的表达临床医学 1 1 材料和方法 3 2 结果 4 3 讨论 6 文2：TNFα在沙漠干热环境下猪腹部肠管火器伤后肝组织中的表达临床医学 7 1 材料和方法 9 2 结果 10 3 讨论 11 参考文摘引言： 13 原创性声明（模板） 14 文章致谢（模板） 15 正文 沙漠干热环境下猪腹部肠管火器伤后肝组织中NFκBTNFα的表达临床医学 文1：沙漠干热环境下猪腹部肠管火器伤后肝组织中NFκBTNFα的表达临床医学 Abstract： Objective To explore the expression of nuclear factor (NF)κB and tumor necrosis factor (TNF)α in hepatic tissue and the mechanism of liver injury following intestinal perforation due to abdominal firearm wound in dryheat environment of desert. Methods A total of 42 ChangBai piglets were equally divided into 7 groups at random: control group, and postwounded 1hour (h) group, 2h group, 4h group, 8h group, 12h group and 24h group. The models of intestinal perforation due to abdominal firearm wound were established in the dryheat environment of desert. Expression of NFκB and TNFα was measured with immunohistochemistry and imaging analysis in all the groups. Serum alanine aminotraferase (ALT) levels were also determined at the same time. The alteration of hepatic tissue was observed under light microscope. Results Expression of hepatic NFκB and TNFα in the post-wounded groups was significantly elevated, compared to that of the control group (P); NFκB presented two peaks in 1h and 4h groups; TNFα appeared and its two peaks belonged to 2h and 8h groups. Levels of serum ALT were also markedly increased, and the two peaks were in 2h and 8h group (P). In all the measurements, the second peak exceed the fit one (P). Under light microscope, hepatocellular edema and metamorphosis was gradually aggravated from 1h group to 2h group. Hepatocellular spot necrosis and piecemeal necrosis were observed in 4h group, and increased from 4h group to 24h group. The liver morphology of the control group was normal under light microscope. Conclusion The expression of NFκB and TNFα in hepatic tissue i