高浓度铅接触对小鼠外周血红细胞形态的影响.docVIP

高浓度铅接触对小鼠外周血红细胞形态的影响 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：高浓度铅接触对小鼠外周血红细胞形态的影响 1 1 材料与方法 3 12 实验方法 3 2 结果 3 3 讨论 4 文2：沙棘汁对铅所致小鼠脑组织生物化学指标的影响 6 1 材料与方法 7 2 结果 9 3 讨论 10 参考文摘引言： 11 原创性声明（模板） 12 文章致谢（模板） 12 正文 高浓度铅接触对小鼠外周血红细胞形态的影响 文1：高浓度铅接触对小鼠外周血红细胞形态的影响 【ABSTRACT】 Objective：To observe the change of red blood cell when poisoned by high concentration lead, and determine the value of abnormal erythrocyte to point out clinical ：Fifteen kunming mice (male and adult) were randomly divided into two groups. Experiment group with ten mice was produced by drinking water(1251mgPb2+/L) to induce lead overload. The control group drinked deionized water. On the 10th ,20th ,30th,40th and 50th day, cut the tail of each mouse to make blood smea, stained the smea by Wrights method, and counted the erythrocyte containing HowellJolly bodies, or containing basophilic stipplings, or showing polychromatophilia, or showing ：The four kinds of abnormal erythrocyte were all increased with the days of lead poisoning(095＜r＜1,P＜001),significantly higher than that of nonexposed mice from 50th ,30th 20th and 10th ：The hypochromic cell is the best examined element to display lead erythrocyte containined HowellJolly bodies doesnt reveal lead poisoning. The basophilic stippling is not accurate at early stage, and we must pay attention to the polychromatic erythrocyte and the hypochromic cell when coidering lead poisoning. 【KEY WORDS】 Lead poisoning;Red blood cell;Abnormal form 铅对红细胞（red blood cell, RBC）具有多方面的毒性作用，可以造成红细胞的各种形态异常。首先，铅是弱的致断剂，可导致DNA损伤[1]，以使RBC细胞质中出现微核，也就是临床检验学中所讲的染色质小体（HowellJolly bodies）。其次，铅抑制RBC中的嘧啶5核苷酸酶（P5N），致使脱核后的RBC中核糖体等不能降解而形成嗜碱性点彩RBC[2]。第三，铅可使血红素合成中的多种巯基酶活性下降[3]，导致血红蛋白生成障碍，最终出现小细胞低色素性贫血。第四，铅可使RBC膜脆性增加、Na+K+ATP酶失活[3]和缺乏能量[2]，因而发生溶血，导致外周血涂片上嗜多色性RBC增加。本研究即是通过小鼠的饮水染毒试验，来了解高浓度铅接触时外周血RBC形态的变化情况，以及这些异常形态对铅中毒的提示作用。 1 材料与方法 11 实验动物 成年雄性（雄激素有利于红细胞的生成）昆明种小鼠15只，体重39~47g，河北北方学院动物室提供。 12 实验方法 121 铅染毒试验 15只小鼠随机分成2组，阴性对照组5只，实验组10只。所有小鼠以混合饲料喂养，适应性喂养一周后，以自由饮水方