FHIT抑癌基因在乳腺癌组织中的表达缺失.docVIP

FHIT抑癌基因在乳腺癌组织中的表达缺失 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：FHIT抑癌基因在乳腺癌组织中的表达缺失 1 1 材料与方法 2 2 结果 3 3 讨论 3 文2：FHIT抑癌基因在乳腺癌组织中的表达缺失 5 1 材料与方法 6 2 结果 7 3 讨论 7 参考文摘引言： 8 原创性声明（模板） 10 文章致谢（模板） 10 正文 FHIT抑癌基因在乳腺癌组织中的表达缺失 文1：FHIT抑癌基因在乳腺癌组织中的表达缺失 【ABSTRACT】 Objective: To investigate the expression characteristics of FHIT protein in breast carcinomas and its relatiohip with clinicopathological paramete. Methods: Expression of FHIT protein in breast carcinoma tissues of 62 cases was detected by SP immunohistochemistry. Results: The positive rate of FHIT protein expression in breast carcinoma tissues was %, and about % cases lost FHIT expression. Negative rate of FHIT expression in early invasive ductal carcinoma in situ group was obviously lower than that in invasive ductal carcinomas group (%), but the difference wasnt significant. Also, loss of FHIT expression in stage Ⅲ～Ⅳ was higher than that in stage Ⅰ～Ⅱ(P=). There was % breast carcinomas with lymph node metastasis lossing FHIT expression, the lossing rate was significantly higher than that in carcinomas without metastasis (P=). FHIT expression had no relatiohip with patients age, distri bution of cance in right or left breast. Conclusion: There was remarkable loss of FHIT protein expression in breast carcinomas. Loss of FHIT expression may be involved in the carcinogenesis of breast or progression of breast carcinomas. 【KEY WORDS】 FHIT; breast carcinoma; Immunohistochemistry 脆性组氨酸三联体(Fragile histidine triad, FHIT)基因是位于染色体上的一个候选抑癌基因，其改变主要见于上皮起源的人类恶性肿瘤。研究已发现FHIT基因在多种肿瘤[1～5]中均有改变且以缺失为主。为了明确FHIT蛋白表达缺失与乳腺癌患者的临床病理参数的相关性及其可能的预后，我们应用免疫组织化学方法对62例乳腺癌组织中FHIT蛋白的表达情况进行了检测。 1 材料与方法 标本来源 收集我院病理教研室～月乳腺癌术后送检标本62例，年龄25～73岁，平均年龄岁。临床分期Ⅰ期16例，Ⅱ期31例，Ⅲ期10例，Ⅳ期5例。所有标本均经10%福尔马林液固定，石蜡包埋，4μｍ连续切片，HE染色，资深病 理学 医师重新光镜下观察确诊。 免疫组织化学染色 采用SP法，主要步骤为：二甲苯脱蜡、梯度酒精脱水，3%双氧水室温处理10min，PBS缓冲液洗涤，专用微波炉进行抗原修复，正常山羊血清37℃封闭、一抗4℃冰箱过夜，次日顺序滴加二抗及三抗，DAB显色10～15min，苏木素复染胞核，脱水透明并用中性树胶封片。同时以已知阳性切片作为阳性对照，用PBS代替一抗作阴性对照。所有试剂均购自北京中杉金桥生物技术有限公司。光镜下