腹腔镜胆囊切除术患者围手术期外周血中IL临床医学.docVIP

腹腔镜胆囊切除术患者围手术期外周血中IL临床医学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：腹腔镜胆囊切除术患者围手术期外周血中IL临床医学 1 1 资料与方法 3 11 一般资料 3 12 麻醉方法与监测 4 13 标本收集与检测方法 4 14 统计学处理 4 2 结果 4 3 讨论 5 文2：肾癌患者外周血中survivinmRNA的表达临床医学 5 1 资料与方法 6 2 结 果 7 1、2、4、5为阴性，第3泳道清晰显示28S、18S两条带 7 3 讨 论 8 参考文摘引言： 11 原创性声明（模板） 12 文章致谢（模板） 12 正文 腹腔镜胆囊切除术患者围手术期外周血中IL临床医学 文1：腹腔镜胆囊切除术患者围手术期外周血中IL临床医学 The Changes of IL6 and TNFα in Peripheral Venous Blood of Laparoscopic Cholecystectomy Patients before and after Operation LI Fulong, LI Jihong，ZHAO Wei Department of Anesthesiology, The Fit Affiliated Hospital, Hebei North Univeity，Zhangjiakou, 075000, Hebei China 【ABSTRACT】 Objective: To compare the effects of laparoscopic cholecystectomy （LC）and open cholecystectomy （OC）on interleukin6（IL6）and tumour necrosis factor alpha（TNFα）in Peripheral venous blood. Methods: Twenty ASA Ⅰ~Ⅱ patients, aged 35~55y,weighing 47~70kg, who had experienced laparoscopic cholecystectomy （LC）were studied. Another 20 patients aged 33~58y,weighing 50~72kg, who had experienced open cholecystectomy （OC）served as control. Peripheral venous blood samples were taken before operation and on the 1st and 3rd postoperation for determination of IL6、TNFα concentratio （ELISA）. Results: IL6、TNFα levels were significantly increased on the 1st postoperative day compared with the baseline values before operation in both groups but there was no significant difference between the two groups. On the 3rd postoperation IL6、TNFα levels returned to preoperative level in group LC while in group OC IL6、TNFα levels remained high. Conclusion: The results showed LC exerts less effects on inflammatory reaction and immune function than that of OC. 【KEY WORDS】 Laparoscopic cholecystectomy （LC）; Open cholecystectomy （OC）; Interleukin6（IL6）; Tumour necrosis factoralpha （TNFα） 手术创伤后单核―巨噬细胞被激活，产生肿瘤坏死因子（tumour necrosis factor; TNF）、白细胞介素6（interleukin6; IL6）等细胞因子，这些促炎细胞因子的过多释放对组织细胞的结构与功能造成损伤，并降低免疫功能，对术后机体的恢复造成不利。有动物实验表明腹腔镜肝切除术对维护机体正常免