炙甘草汤对豚鼠心室肌细胞的电生理影响.docVIP

炙甘草汤对豚鼠心室肌细胞的电生理影响 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：炙甘草汤对豚鼠心室肌细胞的电生理影响 1 1 材料与方法 2 1．4 实验过程和分组 3 2 结果 4 3 讨论 4 文2：炙甘草汤对豚鼠心室肌细胞的电生理影响 5 1 材料与方法 6 1．4 实验过程和分组 7 2 结果 7 3 讨论 7 参考文摘引言： 8 原创性声明（模板） 9 文章致谢（模板） 10 正文 炙甘草汤对豚鼠心室肌细胞的电生理影响 文1：炙甘草汤对豚鼠心室肌细胞的电生理影响 【ABSTRACT】 Objective: To study the electrophysiology effect of Zhigancao Decoction on ventricle muscle cell of :The action potentials were recorded by conventional intracellular microelectrode technique and the amplitude of action potential(APA) and action potentials(APD) were effect of 50% and 90% of duration of action potential(APD50 and APD90) on ventricle muscle cell of rats that were perfused with Zhigancao Decoction was observed as well. Results: (1)For the ventricle muscle cell of rats perfused with 20mg/ml Zhigancao Decoction, electrophysiology had no changes compared with control. (2)For the ventricle muscle cell of rats perfused with 40mg/ml Zhigancao Decoction, the date action potentials(APD) ,90% of duration of action potential(APD90) decreasd(P) compared with control.(3) For the ventricle muscle cell of rats perfused with 80mg/ml Zhigancao Decoction, the date action potentials(APD) decreasd greatly (P),50% and 90% of duration of action potential(APD50 and APD90) decreasd(P) compared with control .Conclusion: Zhigancao Decoction makes the ventricle muscle cell of rats date action potentials(APD) and 50%、 90% of duration of action potential(APD50 and APD90)decrease. 【KEY WORDS】 Zhigancao decoction; Ventricle muscle cell; Electrophysiology 目前，临床上常用炙甘草汤 治疗 心律失常，其抗多种实验性心律失常的研究[1～9]较为广泛。炙甘草汤由甘草、生姜、桂枝、人参、生地黄、阿胶、麦门冬、麻子仁、枣等组成，为汉代张仲景所创，具有滋阴养血，益气温阳，止悸复脉功效，以脉结代，心动悸为证治要点。本实验应用常规的玻璃微电极细胞内记录技术，观察炙甘草汤对豚鼠心室肌细胞电生理影响，报告如下。 1 材料与方法 1．1 标本制备 选用32只～豚鼠，雌雄不拘，随机分为4组，每组8只。击颅致昏后迅速开胸取出心脏，冲洗。至于O2饱和的温度在32±℃的改良Locke液（NaCl /L，KCl ×10-2 mol/L，CaCl2 ×10-2mol/L，NaHCO3 ×10-2 mol/L，葡萄糖，×10-2mol/L，）中。剪去左右心耳，从主动脉进剪，沿右心室外侧缘剪开，暴露左心室，保留乳头肌周围1cm2，其余剪除。制好的标本以不锈钢针固定于灌流槽内的硅胶上，恒温、恒速灌流，流速为5ml/min，在灌流液中持续充以