大鼠肠管外翻模型对葛根素吸收机制的研究.docVIP

大鼠肠管外翻模型对葛根素吸收机制的研究 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：大鼠肠管外翻模型对葛根素吸收机制的研究 1 1 仪器与材料 3 2 方法与结果 3 3 讨论 6 文2：微波辅助提取葛根素的研究 6 1 仪器与药品 7 2 方法与结果 8 3 讨论 10 参考文摘引言： 10 原创性声明（模板） 11 文章致谢（模板） 12 正文 大鼠肠管外翻模型对葛根素吸收机制的研究 文1：大鼠肠管外翻模型对葛根素吸收机制的研究 Abstract：ObjectiveTo develop an accurate, seitive and reliable HPLC method for puerarin, and to study the absorption characteristics of puerarin in different intestinal sectio (jejunum, ileum and colon) and the influence of p-glycoprotein on the absorption everted gut sac was used. Sac containing drug free Krebs solution was immeed into the Krebs solution containing puerarin. The solution iide the sac was collected and drug concentration was determined by HPLC method. Digoxin and verapamil were used to study the influence of p-glycoprotein on the absorption of puerarin. ResultsWith an increase of the concentration of puerarin, puerarin absorption across intestinal membrane was increased in the rat jejunum, ileum and colon, but there exited a saturable traport process for puerarin. With an increase of the concentration of verapamil and digoxin, puerarin absorption across intestinal membrane increased in the rat jejunum, ileum and colon, but when the concentration of digoxin and verapamil was 50 and 200 mg·L-1, the increase had a saturable process. ConclusionThe absorption of puerarin across intestinal sac may be an active traportation mediated by p-glycoprotein traporter. Key words：Puerarin; Everted gut sac; Absorption; Verapamil; Digoxin 葛根素是从豆科植物野葛中提取的有效成分，有扩张冠脉、增加冠脉血流量、降低血压、减慢心率、降低心肌耗氧量等作用［1］。临床上主要用于 治疗 冠心病、心绞痛并发呼吸道和肺部感染、眼底病、心肌炎等多种疾病。葛根素口服生物利用度差［2］是其口服给药的一大不足。药物的生物利用度不高可能是多种因素造成的，其中p-糖蛋白的外排作用是目前研究较多的因素之一。p-糖蛋白底物具有ATP依赖性的药物外排泵的功能，能将细胞内的药物泵出细胞外使细胞内的药物浓度降低［3］。肠管外翻模型在研究p-糖蛋白对药物吸收的影响因素方面 文献 报道较多［4］，因此，本研究旨在运用肠管外翻模型研究葛根素的吸收机制，探讨各肠段和p-糖蛋白抑制剂对葛根素吸收的影响。 1 仪器与材料 仪器Wate高效液相色谱仪（Wate 515泵，2487双波长紫外检测器，Rheodyne 7725i进样阀，CKChrom色谱工作站）（美国Wate公司） 药物葛根素（含量：%，烟台鲁银药业有限公司）；维拉帕米（天津制药厂）；地高辛（美国Sigma公司）；甲醇（色谱纯，淮阴汉邦科技有限公司）